Cloud-clone
SKU:MAB286Ra21
Monoclonal Antibody to Milk Fat Globule EGF Factor 8 (MFGE8)
Monoclonal Antibody to Milk Fat Globule EGF Factor 8 (MFGE8)
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Western blotting: 0.2-2µg/mL;1:500-5000
Immunohistochemistry: 5-20µg/mL;1:50-200
Immunocytochemistry: 5-20µg/mL;1:50-200
Optimal working dilutions must be determined by end user.
Product No.
MAB286Ra21
Organism Species
Rattus norvegicus (Rat).
Source
Monoclonal antibody preparation
Host
Mouse
Potency (Clone Number)
n/a
Ig Isotype
IgG
Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Label
None
Immunogen
n/a
Buffer Formulation
PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Traits
Liquid
Concentration
1mg/mL
Organism Species More
n/a
Applications
WB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
UOM
20µl 100µl 200µl 1ml 10ml
SPECIFITY
The antibody is a mouse monoclonal antibody raised against MFGE8. It has been selected for its ability to recognize MFGE8 in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.2-2µg/mL;1:500-5000
Immunohistochemistry: 5-20µg/mL;1:50-200
Immunocytochemistry: 5-20µg/mL;1:50-200
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
| Magazine | Citations |
| Fertility and Sterility | Tumor necrosis factor α up-regulates endometrial milk fat globule-epidermal growth factor 8 protein production via nuclear factor κB activation, resulting in cell migration of epithelial cells. Pubmed: 24262600 |
| U.S. Patent Application | Biomarker for amyloid-b-related neurological disorders US20130302838 |
| IUBMB Life. | Elevated serum milk fat globule-epidermal growth factor 8 levels in type 2 diabetic patients are suppressed by overweight or obese status. pubmed:28067023 |
| Journal of diabetes investigation | Circulating milk fat globule-epidermal growth factor 8 levels are increased in pregnancy and gestational diabetes mellitus. pubmed:28035763 |
| The Journal of Steroid Biochemistry and Molecular Biology | Modifications of Western-type diet regarding protein, fat and sucrose levels as modulators of steroid metabolism and activity in liver pubmed:27471150 |
| Journal of Steroid Biochemistry and Molecular Biology | Depressive-like effect of prenatal exposure to DDT involves global DNA hypomethylation and impairment of GPER1/ESR1 protein levels but not ESR2 and AHR/ARNT signaling pubmed:28263910 |
| Oncotarget | Pantoprazole blocks the JAK2/STAT3 pathway to alleviate skeletal muscle wasting in cancer cachexia by inhibiting inflammatory response. pubmed:28489606 |
| Molecular and Cellular Neuroscience | TGF-β1 enhances phagocytic removal of neuron debris and neuronal survival by olfactory ensheathing cells via integrin/MFG-E8 signaling pathway 10.1016/j.mcn.2017.08.006 |
| Amyloid | Needle-shaped amyloid deposition in rat mammary gland: evidence of a novel amyloid fibril protein Pubmed: 31615282 |
| clinical and translational medicine | Milk fat globule EGF factor 8 restores mitochondrial function via integrin©\medicated activation of the FAK©\STAT3 signaling pathway in acute pancreatitis 33634976 |
| Catalog No. | Related products for research use of Rattus norvegicus (Rat) Organism species | Applications (RESEARCH USE ONLY!) |
| RPB286Ra01 | Recombinant Milk Fat Globule EGF Factor 8 (MFGE8) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB286Ra01 | Polyclonal Antibody to Milk Fat Globule EGF Factor 8 (MFGE8) | WB; IHC; ICC; IP. |
| MAB286Ra21 | Monoclonal Antibody to Milk Fat Globule EGF Factor 8 (MFGE8) | WB; IHC; ICC; IP. |
| SEB286Ra | ELISA Kit for Milk Fat Globule EGF Factor 8 (MFGE8) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB286Ra | Magnetic Luminex Assay Kit for Milk Fat Globule EGF Factor 8 (MFGE8) ,etc. | Magnetic Luminex Assay for Antigen Detection. |
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