Cloud-clone
SKU:MAB373Ra21
Monoclonal Antibody to Defensin Beta 1 (DEFb1)
Monoclonal Antibody to Defensin Beta 1 (DEFb1)
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Western blotting: 0.2-2µg/mL;1:500-5000
Immunohistochemistry: 5-20µg/mL;1:50-200
Immunocytochemistry: 5-20µg/mL;1:50-200
Optimal working dilutions must be determined by end user.
Product No.
MAB373Ra21
Organism Species
Rattus norvegicus (Rat).
Source
Monoclonal antibody preparation
Host
Mouse
Potency (Clone Number)
C3
Ig Isotype
IgG
Purification
Protein A + Protein G affinity chromatography
Label
None
Immunogen
RPB373Ra01-Recombinant Defensin Beta 1 (DEFb1)
Buffer Formulation
0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Traits
Liquid
Concentration
1mg/mL
Organism Species More
n/a
Applications
WB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
UOM
20µl 100µl 200µl 1ml 10ml
SPECIFITY
The antibody is a mouse monoclonal antibody raised against DEFb1. It has been selected for its ability to recognize DEFb1 in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.5-2µg/mL
Immunohistochemistry: 5-20µg/mL
Immunocytochemistry: 5-20µg/mL
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
| Magazine | Citations |
| Appl Environ Microbiol. | Modulation of Porcine β-Defensins 1 and 2 upon Individual and Combined Fusarium Toxin Exposure in a Swine Jejunal Epithelial Cell Line Asm: Source |
| British Journal of Nutrition | Identification of sociodemographic and clinical factors associated with the levels of human β-defensin-1 and human β-defensin-2 in the human milk of Han Chinese Pubmed: 24124699 |
| International Journal of Dairy Technology | Concentration characteristics of bovine β-defensin 1 and 2 in fresh bovine milk and infant formula Doi: 10.1111 |
| Molecular Nutrition Food Research | Green tea polyphenol epigallocatechin-3-gallate improves epithelial barrier function by inducing the productionof antimicrobial peptide pBD-1 and pBD-2 in monolayers of porcine intestinal epithelial IPEC-J2 cells. Pubmed:26991948 |
| Frontiers in Microbiology | Swine-Derived Probiotic Lactobacillus plantarum Inhibits Growth and Adhesion of Enterotoxigenic Escherichia coli and Mediates Host Defense Pubmed:29997590 |
| International Immunopharmacology | Caprylic acid and nonanoic acid upregulate endogenous host defense peptides to enhance intestinal epithelial immunological barrier function via histone deacetylase … Pubmed: 30342347 |
| cyberleninka | EFFECT OF NIOSOMAL ANTIMICROBIAL PEPTIDE HBD-1 ON THE HEALING RATE OF INFECTED WOUNDS IN RATS |
| Fundamental & clinical pharmacology | Effect of antimicrobial peptides HNP‐1 and hBD‐1 on Staphylococcus aureus strains in vitro and in vivo Pubmed: 31313350 |
| Cytokine | Levels of pro-and anti-inflammatory cytokines in cystic fibrosis patients with or without gingivitis Pubmed: 31927460 |
| Animals | Fat Encapsulation Reduces Diarrhea in Piglets Partially by Repairing the Intestinal Barrier and Improving Fatty Acid Transport 33375218 |
| Sci Rep | Human beta defensin levels and vaginal microbiome composition in post-menopausal women diagnosed with lichen sclerosus 34362937 |
| J Mol Histol | Isolation, culture, and identification of ceruminous gland cells Pubmed:35113280 |
| Catalog No. | Related products for research use of Rattus norvegicus (Rat) Organism species | Applications (RESEARCH USE ONLY!) |
| RPB373Ra01 | Recombinant Defensin Beta 1 (DEFb1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB373Ra01 | Polyclonal Antibody to Defensin Beta 1 (DEFb1) | WB; IHC; ICC; IP. |
| PAB373Ra51 | Polyclonal Antibody to Defensin Beta 1 (DEFb1) | WB; IHC; ICC; IP. |
| LAB373Ra71 | Biotin-Linked Polyclonal Antibody to Defensin Beta 1 (DEFb1) | WB; IHC; ICC. |
| MAB373Ra21 | Monoclonal Antibody to Defensin Beta 1 (DEFb1) | WB; IHC; ICC; IP. |
| SEB373Ra | ELISA Kit for Defensin Beta 1 (DEFb1) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB373Ra | Magnetic Luminex Assay Kit for Defensin Beta 1 (DEFb1) ,etc. | Magnetic Luminex Assay for Antigen Detection. |
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