Cloud-clone
SKU:MAB449Ra21
Monoclonal Antibody to Enolase 1 (ENO1)
Monoclonal Antibody to Enolase 1 (ENO1)
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Western blotting: 0.2-2µg/mL;1:500-5000
Immunohistochemistry: 5-20µg/mL;1:50-200
Immunocytochemistry: 5-20µg/mL;1:50-200
Optimal working dilutions must be determined by end user.
Product No.
MAB449Ra21
Organism Species
Rattus norvegicus (Rat).
Source
Monoclonal antibody preparation
Host
Mouse
Potency (Clone Number)
n/a
Ig Isotype
IgG
Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Label
None
Immunogen
n/a
Buffer Formulation
PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Traits
Liquid
Concentration
1mg/mL
Organism Species More
n/a
Applications
WB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
UOM
20µl 100µl 200µl 1ml 10ml
SPECIFITY
The antibody is a mouse monoclonal antibody raised against ENO1. It has been selected for its ability to recognize ENO1 in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.2-2µg/mL;1:500-5000
Immunohistochemistry: 5-20µg/mL;1:50-200
Immunocytochemistry: 5-20µg/mL;1:50-200
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
| Magazine | Citations |
| Analytical Chemistry | Diagnostic detection of human lung cancer-associated antigen using a gold nanoparticle-based electrochemical immunosensor PubMed: 20557064 |
| Translational Research | Proteomic analysis of fine-needle aspiration in differential diagnosis of thyroid nodules Pubmed:27172385 |
| Journal of Proteomics | Putative salivary biomarkers useful to differentiate patients with fibromyalgia Pubmed:29654921 |
| BioMed Research International | The Diagnostic Accuracy of Combined Enolase/Cr, CA125, and CA19-9 in the Detection of Endometriosis Pubmed: 33062681 |
| Cancer Cell International | Bufalin Induced Mitochondrial Dysfunction Promotes Apoptosis of Glioma Cells by Regulating Annexin A2 and DRP1 Proteins |
| Cancer Cell Int | Bufalin induces mitochondrial dysfunction and promotes apoptosis of glioma cells by regulating Annexin A2 and DRP1 protein expression 34376212 |
| Clin Proteomics | Serum proteomics unveil characteristic protein diagnostic biomarkers and signaling pathways in patients with esophageal squamous cell carcinoma Pubmed:35610567 |
| Catalog No. | Related products for research use of Rattus norvegicus (Rat) Organism species | Applications (RESEARCH USE ONLY!) |
| RPB449Ra01 | Recombinant Enolase 1 (ENO1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB449Ra01 | Polyclonal Antibody to Enolase 1 (ENO1) | WB; IHC; ICC; IP. |
| MAB449Ra21 | Monoclonal Antibody to Enolase 1 (ENO1) | WB; IHC; ICC; IP. |
| SEB449Ra | ELISA Kit for Enolase 1 (ENO1) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB449Ra | Magnetic Luminex Assay Kit for Enolase 1 (ENO1) ,etc. | Magnetic Luminex Assay for Antigen Detection. |
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