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SKU:PAM285Ra01
Polyclonal Antibody to Steroid 5 Alpha Reductase 2 (SRD5a2)
Polyclonal Antibody to Steroid 5 Alpha Reductase 2 (SRD5a2)
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Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.
Product No.
PAM285Ra01
Organism Species
Rattus norvegicus (Rat).
Source
Polyclonal antibody preparation
Host
Rabbit
Potency
n/a
Ig Type
IgG
Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Label
None
Immunogen
n/a
Buffer Formulation
PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Traits
Liquid
Concentration
500µg/mL
Organism Species More
n/a
Applications
WB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
UOM
20µl 100µl 200µl 1ml 10ml
SPECIFITY
The antibody is a rabbit polyclonal antibody raised against SRD5a2. It has been selected for its ability to recognize SRD5a2 in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
| Magazine | Citations |
| Evid Based Complement Alternat Med. | Seoritae extract reduces prostate weight and suppresses prostate cell proliferation in a rat model of benign prostate hyperplasia Pubmed:24701239 |
| Neuropharmacology. | TSPO activation modulates the effects of high pressure in a rat ex vivo glaucoma model. pubmed:27596950 |
| Journal of Pharmacognosy and Phytochemistry | Effects of ethanolic extracts of Cola millenii K. Schum seed on biochemical and toxicological indices of male wistar albino rats 1616357 |
| Nutrition Research and Practice | Corni Fructus attenuates testosterone-induced benign prostatic hyperplasia by suppressing 5α-reductase and androgen receptor expression in rats 10.4162:nrp.2018.12.e36 |
| Eur J Pharmacol | Yohimbine hydrochloride inhibits benign prostatic hyperplasia by downregulating steroid 5α-reductase type 2 34265299 |
| Catalog No. | Related products for research use of Rattus norvegicus (Rat) Organism species | Applications (RESEARCH USE ONLY!) |
| RPM285Ra01 | Recombinant Steroid 5 Alpha Reductase 2 (SRD5a2) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAM285Ra01 | Polyclonal Antibody to Steroid 5 Alpha Reductase 2 (SRD5a2) | WB; IHC; ICC; IP. |
| MAM285Ra21 | Monoclonal Antibody to Steroid 5 Alpha Reductase 2 (SRD5a2) | WB; IHC; ICC; IP. |
| SEM285Ra | ELISA Kit for Steroid 5 Alpha Reductase 2 (SRD5a2) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMM285Ra | Magnetic Luminex Assay Kit for Steroid 5 Alpha Reductase 2 (SRD5a2) ,etc. | Magnetic Luminex Assay for Antigen Detection. |
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