Cloud-clone
SKU:PAP553Mu01
Polyclonal Antibody to Wingless Type MMTV Integration Site Family, Member 10B (WNT10B)
Polyclonal Antibody to Wingless Type MMTV Integration Site Family, Member 10B (WNT10B)
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Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.
Product No.
PAP553Mu01
Organism Species
Mus musculus (Mouse).
Source
Polyclonal antibody preparation
Host
Rabbit
Potency
n/a
Ig Type
IgG
Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Label
None
Immunogen
n/a
Buffer Formulation
PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Traits
Liquid
Concentration
500µg/mL
Organism Species More
n/a
Applications
WB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
UOM
20µl 100µl 200µl 1ml 10ml
SPECIFITY
The antibody is a rabbit polyclonal antibody raised against WNT10B. It has been selected for its ability to recognize WNT10B in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
| Magazine | Citations |
| Arthritis & Rheumatology | CTLA-4Ig–Induced T Cell Anergy Promotes Wnt-10b Production and Bone Formation in a Mouse Model Onlinelibrary: art.38319 |
| Annals of The New York Academy of Sciences | CTLA‐4Ig (abatacept) balances bone anabolic effects of T cells and Wnt‐10b with antianabolic effects of osteoblastic sclerostin Pubmed:29500936 |
| International Journal of Medical Sciences | Hypoalbuminemia differently affects the serum bone turnover markers in hemodialysis patients Pubmed: 31839746 |
| Food & Function | Comparative study of DHA-enriched phosphatidylcholine and EPA-enriched phosphatidylcholine on ameliorating high bone turnover via regulation of the … Pubmed: 33140795 |
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