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SKU:PAB480Hu03

Polyclonal Antibody to Mannose Binding Lectin (MBL)

Polyclonal Antibody to Mannose Binding Lectin (MBL)

Regular price $332.00 USD
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UOM
Concentration
Organism species

Western blotting: 1-5µg/mL
Immunocytochemistry in formalin fixed cells: 5-20µg/mL
Immunohistochemistry in formalin fixed frozen section: 5-20µg/mL
Immunohistochemistry in paraffin section: 5-20µg/mL
Enzyme-linked Immunosorbent Assay: 0.05-2µg/mL
Optimal working dilutions must be determined by end user.

Product No.

PAB480Hu03

Organism Species

Homo sapiens (Human).

Source

Polyclonal antibody preparation

Host

Rabbit

Potency

n/a

Ig Type

IgG

Purification

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Label

None

Immunogen

RPB480Hu03-Recombinant Mannose Binding Lectin (MBL)

Buffer Formulation

0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.

Traits

Liquid

Concentration

500µg/mL

Organism Species More

n/a

Applications

WB; IHC; ICC; IP.

If the antibody is used in flow cytometry, please check FCM antibodies.

UOM

20µl 100µl 200µl 1ml 10ml

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against MBL. It has been selected for its ability to recognize MBL in immunohistochemical staining and western blotting.

USAGE

Western blottin:g: 0.5-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Magazine Citations
American Journal of Nephrology Analysis of the Urine Proteome of Human Contrast-Induced Kidney Injury Using Two-Dimensional Fluorescence Differential Gel Electrophoresis/Matrix-Assisted Laser Desorption Time-of-Flight Mass Spectrometry/Liquid Chromatography Mass Spectrometry. Karger: 000255439
Current Eye Research Serum Levels and H/L Gene Polymorphism of Mannose-Binding Lectin in Primary Open Angle Glaucoma Ingenta: art00007
Journal of Clinical Immunology Complement activation contributes to the injury and outcome of kidney in human anti-glomerular basement membrane disease. PubMed: 22941511
Clinical and Experimental Immunology Urinary mannose-binding lectin is a biomarker for predicting the progression of immunoglobulin (Ig)A nephropathy PubMed: PMC3406374
Clin J Am Soc Nephrol. Alternative Complement Pathway Activation Products in Urine and Kidneys of Patients with ANCA-Associated GN Pubmed: 24115193
ACTA TROPICA Novel findings on the role of ficolins and colectins in the innate response against Leishmania braziliensis Pubmed: 32827456
PLoS One Complement activation profile of patients with primary focal segmental glomerulosclerosis Pubmed: 32569286
LIFE SCIENCES The lectin pathway of complement and the initial recognition of Leishmania infantum promastigotes 34242658
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