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SKU:PAB053Ra01
Polyclonal Antibody to Cytochrome P450 7A1 (CYP7A1)
Polyclonal Antibody to Cytochrome P450 7A1 (CYP7A1)
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Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.
Product No.
PAB053Ra01
Organism Species
Rattus norvegicus (Rat).
Source
Polyclonal antibody preparation
Host
Rabbit
Potency
n/a
Ig Type
IgG
Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Label
None
Immunogen
RPB053Ra01-Recombinant Cytochrome P450 7A1 (CYP7A1)
Buffer Formulation
0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Traits
Liquid
Concentration
500µg/mL
Organism Species More
n/a
Applications
WB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
UOM
20µl 100µl 200µl 1ml 10ml
SPECIFITY
The antibody is a rabbit polyclonal antibody raised against CYP7A1. It has been selected for its ability to recognize CYP7A1 in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.5-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
| Magazine | Citations |
| Cell Reports | Parabacteroides distasonis Alleviates Obesity and Metabolic Dysfunctions via Production of Succinate and Secondary Bile Acids Pubmed: 30605678 |
| Journal of Functional Foods | Hyperglycemia exacerbates dyslipidemia-induced changes in uptake, synthesis, and transporters of bile acids in rats: Assessment of restorative potentials of ALA and?… Doi: 10.1016/j.jff.2019.01.038 |
| Journal of Agriculture and Food Research | Withaferin-A down-regulate enterohepatic circulation of bile acids: An insight from a hyperlipidemic rat model |
| Catalog No. | Related products for research use of Rattus norvegicus (Rat) Organism species | Applications (RESEARCH USE ONLY!) |
| RPB053Ra01 | Recombinant Cytochrome P450 7A1 (CYP7A1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB053Ra01 | Polyclonal Antibody to Cytochrome P450 7A1 (CYP7A1) | WB; IHC; ICC; IP. |
| MAB053Ra21 | Monoclonal Antibody to Cytochrome P450 7A1 (CYP7A1) | WB; IHC; ICC; IP. |
| SEB053Ra | ELISA Kit for Cytochrome P450 7A1 (CYP7A1) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB053Ra | Magnetic Luminex Assay Kit for Cytochrome P450 7A1 (CYP7A1) ,etc. | Magnetic Luminex Assay for Antigen Detection. |
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