Cloud-clone
SKU:MAB357Ra21
Monoclonal Antibody to Extracellular Signal Regulated Kinase 1 (ERK1)
Monoclonal Antibody to Extracellular Signal Regulated Kinase 1 (ERK1)
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Western blotting: 0.2-2µg/mL;1:500-5000
Immunohistochemistry: 5-20µg/mL;1:50-200
Immunocytochemistry: 5-20µg/mL;1:50-200
Optimal working dilutions must be determined by end user.
Product No.
MAB357Ra21
Organism Species
Rattus norvegicus (Rat).
Source
Monoclonal antibody preparation
Host
Mouse
Potency (Clone Number)
n/a
Ig Isotype
IgG
Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Label
None
Immunogen
n/a
Buffer Formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
Traits
Liquid
Concentration
1mg/mL
Organism Species More
n/a
Applications
WB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
UOM
20µl 100µl 200µl 1ml 10ml
SPECIFITY
The antibody is a mouse monoclonal antibody raised against ERK1. It has been selected for its ability to recognize ERK1 in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.2-2µg/mL;1:500-5000
Immunohistochemistry: 5-20µg/mL;1:50-200
Immunocytochemistry: 5-20µg/mL;1:50-200
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
| Magazine | Citations |
| Graefe's Archive for Clinical and Experimental Ophthalmology | Role of RhoA/MERK1/ERK1/2/iNOS signaling in ocular ischemic syndrome pubmed:27538909 |
| International Journal of Reproductive Medicine | Gonadotropin Stimulation Has Only a Limited Effect on the Concentration of Follicular Fluid Signalling Proteins: An Antibody Array Analysis 33575317 |
| Sci Rep | Curcumin derivative ST09 modulates the miR-199a-5p/DDR1 axis and regulates proliferation and migration in ovarian cancer cells 34837026 |
| Catalog No. | Related products for research use of Rattus norvegicus (Rat) Organism species | Applications (RESEARCH USE ONLY!) |
| RPB357Ra01 | Recombinant Extracellular Signal Regulated Kinase 1 (ERK1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB357Ra01 | Polyclonal Antibody to Extracellular Signal Regulated Kinase 1 (ERK1) | WB; IHC; ICC; IP. |
| LAB357Ra81 | FITC-Linked Polyclonal Antibody to Extracellular Signal Regulated Kinase 1 (ERK1) | WB; IHC; ICC; IF. |
| MAB357Ra21 | Monoclonal Antibody to Extracellular Signal Regulated Kinase 1 (ERK1) | WB; IHC; ICC; IP. |
| SEB357Ra | ELISA Kit for Extracellular Signal Regulated Kinase 1 (ERK1) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB357Ra | Magnetic Luminex Assay Kit for Extracellular Signal Regulated Kinase 1 (ERK1) ,etc. | Magnetic Luminex Assay for Antigen Detection. |
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