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SKU:PAA937Hu02

Polyclonal Antibody to Nephrin (NPHN)

Polyclonal Antibody to Nephrin (NPHN)

Regular price $314.00 USD
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UOM
Concentration
Organism species

Western blotting: 0.5-2µg/mL;1:500-2000
Immunohistochemistry: 5-20µg/mL;1:50-200
Immunocytochemistry: 5-20µg/mL;1:50-200
Optimal working dilutions must be determined by end user.

Product No.

PAA937Hu02

Organism Species

Homo sapiens (Human).

Source

Polyclonal antibody preparation

Host

Rabbit

Potency

n/a

Ig Type

IgG

Purification

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Label

None

Immunogen

RPA937Hu02-Recombinant Nephrin (NPHN)

Buffer Formulation

PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.

Traits

Liquid

Concentration

0.5mg/ml

Organism Species More

Mus musculus (Mouse), Rattus norvegicus (Rat)

Applications

WB; IHC; ICC; IP.

If the antibody is used in flow cytometry, please check FCM antibodies.

UOM

20µl 100µl 200µl 1ml 10ml

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against NPHN. It has been selected for its ability to recognize NPHN in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.01-2µg/mL
Immunohistochemistry: 5-20µg/mL
Immunocytochemistry: 5-20µg/mL
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Magazine Citations
Yonsei medical journal The Level of Serum and Urinary Nephrin in Normal Pregnancy and Pregnancy with Subsequent Preeclampsia. pubmed:28120572
Experimental and molecular pathology Alteration of Connexin43 expression in a rat model of obesity-related glomerulopathy. pubmed:29246788
British Journal of Haematology  Renal protection by atorvastatin in a murine model of sickle cell nephropathy Pubmed:29527679
Journal of Clinical Medicine Multiplex Bead Array Assay of a Panel of Circulating Cytokines and Growth Factors in Patients with Albuminuric and Non-AlbuminuricDiabetic Kidney Disease Pubmed: 32961903
Disease Markers Urinary Matrix Metalloproteinase-9 and Nephrin in Idiopathic Membranous Nephropathy: A Cross-Sectional Study 34707724
Biomed Rep Early biomarkers of nephrotoxicity associated with the use of anti-VEGF drugs Pubmed:35620307
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