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SKU:PAL604Co01

Polyclonal Antibody to High Temperature Requirement Factor A1 (HTRA1)

Polyclonal Antibody to High Temperature Requirement Factor A1 (HTRA1)

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UOM
Concentration
Organism species

Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.

Product No.

PAL604Co01

Organism Species

Escherichia coli.

Source

Polyclonal antibody preparation

Host

Rabbit

Potency

n/a

Ig Type

IgG

Purification

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Label

None

Immunogen

n/a

Buffer Formulation

PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.

Traits

Liquid

Concentration

500µg/mL

Organism Species More

n/a

Applications

WB; IHC; ICC; IP.

If the antibody is used in flow cytometry, please check FCM antibodies.

UOM

20µl 100µl 200µl 1ml 10ml

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against HTRA1. It has been selected for its ability to recognize HTRA1 in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Magazine Citations
Placenta Alterations of maternal plasma HTRA1 level in preeclampsia complicated by IUGR PubMed: 23083999
Placenta Upregulation of HtrA4 in the placentas of patients with severe pre-eclampsia PubMed: 22964307
Exp Gerontol Association of frailty with the serine protease HtrA1 in older adults Pubmed:27058767
International Journal of Clinical and Experimental Pathology HtrA1 up-regulates expression of MMPs via Erk1/2/Rock-dependent pathways files:ijcep0069706.pdf
Pregnancy Hypertension Association between first trimester plasma htra1 level and subsequent preeclampsia: a possible early marker?
American journal of translational research HtrA1 upregulates the expression of ADAMTS-5 in HNPCs via the ERK/NF-κB/JNK signaling pathway Pubmed: 31497227
J Matern Fetal Neonatal Med First trimester HtrA1 maternal plasma level and spontaneous preterm birth Pubmed: 32102578
BMC Geriatr Cross-sectional associations among P3NP, HtrA, Hsp70, Apelin and sarcopenia in Taiwanese population 33743591
Catalog No. Related products for research use of Escherichia coli Organism species Applications (RESEARCH USE ONLY!)
RPL604Co02 Recombinant High Temperature Requirement Factor A1 (HTRA1) Positive Control; Immunogen; SDS-PAGE; WB.
RPL604Co01 Recombinant High Temperature Requirement Factor A1 (HTRA1) Positive Control; Immunogen; SDS-PAGE; WB.
PAL604Co01 Polyclonal Antibody to High Temperature Requirement Factor A1 (HTRA1) WB; IHC; ICC; IP.
PAL604Co02 Polyclonal Antibody to High Temperature Requirement Factor A1 (HTRA1) WB
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