Cloud-clone
SKU:MAA424Hu21
Monoclonal Antibody to Octamer Binding Transcription Factor 4 (OCT4)
Monoclonal Antibody to Octamer Binding Transcription Factor 4 (OCT4)
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Western blotting: 0.2-2µg/mL;1:500-5000
Immunohistochemistry: 5-20µg/mL;1:50-200
Immunocytochemistry: 5-20µg/mL;1:50-200
Optimal working dilutions must be determined by end user.
Product No.
MAA424Hu21
Organism Species
Homo sapiens (Human).
Source
Monoclonal antibody preparation
Host
Mouse
Potency (Clone Number)
C6
Ig Isotype
IgG2a Kappa
Purification
Protein A + Protein G affinity chromatography
Label
None
Immunogen
RPA424Hu01-Recombinant Octamer Binding Transcription Factor 4 (OCT4)
Buffer Formulation
0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Traits
Liquid
Concentration
1mg/ml
Organism Species More
n/a
Applications
WB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
UOM
20µl 100µl 200µl 1ml 10ml
SPECIFITY
The antibody is a mouse monoclonal antibody raised against OCT4. It has been selected for its ability to recognize OCT4 in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.5-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
| Magazine | Citations |
| Analytical Methods | A universal glucometer-based biosensor for portable and quantitative detection of transcription factors Rsc: Source |
| Oncology Letters | Upregulation of stem cell markers ALDH1A1 and OCT4 as potential biomarkers for the early detection of cervical carcinoma Doi: 10.3892/ol.2018.9381 |
| Stem Cell Research | Generation of two induced pluripotent stem cell lines NCCSi005A and NCCSi006A from CD4+ T cells of healthy individuals of Indian origin |
| 博士论文 | Experimental and Modeling Framework for the Production of Cell and Protein-Based Biopharmaceutical Products |
| Stem Cell Research | Generation and characterization of three clones (NCCSi007A, NCCSi007B and NCCSi007C) of an integration free induced Pluripotent Stem Cell line from a … Pubmed: 31862609 |
| J Biochem Mol Toxicol | Studies on male gonadal toxicity of bisphenol A in diabetic rats: An example of exacerbation effect Pubmed:35187753 |
| Catalog No. | Related products for research use of Homo sapiens (Human) Organism species | Applications (RESEARCH USE ONLY!) |
| RPA424Hu01 | Recombinant Octamer Binding Transcription Factor 4 (OCT4) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA424Hu01 | Polyclonal Antibody to Octamer Binding Transcription Factor 4 (OCT4) | WB; IHC; ICC; IP. |
| MAA424Hu21 | Monoclonal Antibody to Octamer Binding Transcription Factor 4 (OCT4) | WB; IHC; ICC; IP. |
| SEA424Hu | ELISA Kit for Octamer Binding Transcription Factor 4 (OCT4) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA424Hu | Magnetic Luminex Assay Kit for Octamer Binding Transcription Factor 4 (OCT4) ,etc. | Magnetic Luminex Assay for Antigen Detection. |
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