Cloud-clone
SKU:PAD066Hu03
Polyclonal Antibody to Apelin (APLN)
Polyclonal Antibody to Apelin (APLN)
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Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.
Product No.
PAD066Hu03
Organism Species
Homo sapiens (Human).
Source
Polyclonal antibody preparation
Host
Rabbit
Potency
n/a
Ig Type
IgG
Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Label
None
Immunogen
n/a
Buffer Formulation
PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Traits
Liquid
Concentration
500µg/mL
Organism Species More
n/a
Applications
WB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
UOM
20µl 100µl 200µl 1ml 10ml
SPECIFITY
The antibody is a rabbit polyclonal antibody raised against APLN. It has been selected for its ability to recognize APLN in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
| Magazine | Citations |
| Clin Exp Hypertens. | Individual and Concomitant Effects of Cardioprotective Programs on Cardiac Apelinergic System and Oxidative State in └-NAME-Induced Hypertension Informahealthcare: Source |
| African Journal of Pharmacy and Pharmacology | Cardioprotective effects of aerobic regular exercise against doxorubicin-induced oxidative stress in rat. Academicjournals: Source |
| Zahedan Journal of research in Medical Sciences | Improvement of Kidney Apelin and Apelin Receptor in Nitro-L-Arginine-Methyl Ester Induced Hypertension Rats Zjrms: Source |
| Endocr J. | CTRP3 modulates the expression and secretion of adipokines in 3T3-L1 adipocytes Pubmed:25168658 |
| Mol Metab | TIMP3 interplays with apelin to regulate cardiovascular metabolism in hypercholesterolemic mice PubMed: 26500845 |
| Journal of medicinal food | Regulation of GIP and Ghrelin in Healthy Subjects Fed on Sun-Dried Raisins: A Pilot Study with a Crossover Trial Design. pubmed:28170279 |
| Arch Physiol Biochem | Associations of apelin, leptin, irisin, ghrelin, insulin, glucose levels, and lipid parameters with physical activity during eight weeks of regular exercise training Pubmed: 31290696 |
| preprints | Serum Apelin and Galactin-3 in Preeclampsia in Iraq Pubmed: 32538210 |
| Journal of Diabetes Research | The Potential Role of Chemerin, Lipocalin 2, and Apelin in the Diagnosis and Pathophysiology of Gestational Diabetes Mellitus |
| Catalog No. | Related products for research use of Homo sapiens (Human) Organism species | Applications (RESEARCH USE ONLY!) |
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