Western blotting: 0.5-2µg/mL ;Immunohistochemistry: 5-20µg/mL ;Immunocytochemistry: 5-20µg/mL ;Optimal working dilutions must be determined by end user.
Product No.
PAB249Hu01
Organism Species
Homo sapiens (Human).
Source
Polyclonal antibody preparation
Host
Rabbit
Potency
n/a
Ig Type
IgG
Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Label
None
Immunogen
RPB249Hu01-Recombinant Tumor Necrosis Factor Receptor Superfamily, Member 5 (CD40)
Buffer Formulation
PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Traits
Liquid
Concentration
500µg/mL
Organism Species More
n/a
Applications
WB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
UOM
20µl 100µl 200µl 1ml 10ml
SPECIFITY
The antibody is a rabbit polyclonal antibody raised against CD40. It has been selected for its ability to recognize CD40 in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.5-2µg/mL Immunohistochemistry: 5-20µg/mL Immunocytochemistry: 5-20µg/mL Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Magazine
Citations
american journal of physiology-renal physiology
The expression of XBP1s in B lymphocytes is critical for pristane-induced lupusnephritis in micePubmed: 32249615
Catalog No.
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