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SKU:PAB283Ga01
Polyclonal Antibody to Epithelial Cell Adhesion Molecule (EPCAM)
Polyclonal Antibody to Epithelial Cell Adhesion Molecule (EPCAM)
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Western blotting: 0.5-2µg/mL
;Immunohistochemistry: 5-20µg/mL
;Immunocytochemistry: 5-20µg/mL
;Optimal working dilutions must be determined by end user.
Product No.
PAB283Ga01
Organism Species
Chicken (Gallus).
Source
Polyclonal antibody preparation
Host
Rabbit
Potency
n/a
Ig Type
IgG
Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Label
None
Immunogen
RPB283Ga01-Recombinant Epithelial Cell Adhesion Molecule (EPCAM)
Buffer Formulation
PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Traits
Liquid
Concentration
0.79mg/ml
Organism Species More
n/a
Applications
WB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
UOM
20µl 100µl 200µl 1ml 10ml
SPECIFITY
The antibody is a rabbit polyclonal antibody raised against EPCAM. It has been selected for its ability to recognize EPCAM in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.01-3µg/mL;
Immunohistochemistry: 5-30µg/mL;
Immunocytochemistry: 5-30µg/mL;
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
| Magazine | Citations |
| Sensors and Actuators B: Chemical | Epithelial cancer biomarker EpCAM determination in peripheral blood samples using a microfluidic immunosensor based in silver nanoparticles as platform Science: Article |
| Microchemical Journal | Nanostructured platform integrated into a microfluidic immunosensor coupled to laser-induced fluorescence for the epithelial cancer biomarker determination science:S0026265X16300091 |
| Arab Journal of Gastroenterology | Role of several cytokines and adhesion molecules in the diagnosis and prediction of survival ofhepatocellular carcinoma. pubmed:27916547 |
| Clinica Chimica Acta | Integrated bio-affinity nano-platform into a microfluidic immunosensor based on monoclonal bispecific trifunctional antibodies for the electrochemical determination of … pubmed:27836687 |
| Clinica Chimica Acta | Integrated bio-affinity nano-platform into a microfluidic immunosensor based on monoclonal bispecific trifunctional antibodies for the electrochemical determination of epithelial cancer biomarker 10.1016/j.cca.2016.11.012 |
| Catalog No. | Related products for research use of Chicken (Gallus) Organism species | Applications (RESEARCH USE ONLY!) |
| RPB283Ga01 | Recombinant Epithelial Cell Adhesion Molecule (EPCAM) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB283Ga01 | Polyclonal Antibody to Epithelial Cell Adhesion Molecule (EPCAM) | WB; IHC; ICC; IP. |
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