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SKU:PAB081Hu02

Polyclonal Antibody to Heat Shock 70kDa Protein 1A (HSPA1A)

Polyclonal Antibody to Heat Shock 70kDa Protein 1A (HSPA1A)

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UOM
Concentration
Organism species

Western blotting: 0.5-2µg/mL;
;Immunohistochemistry: 5-20µg/mL;
;Immunocytochemistry: 5-20µg/mL;
;Optimal working dilutions must be determined by end user.

Product No.

PAB081Hu02

Organism Species

Homo sapiens (Human) .

Source

Polyclonal antibody preparation

Host

Rabbit

Potency

n/a

Ig Type

IgG

Purification

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Label

None

Immunogen

RPB081Hu02-Recombinant Heat Shock 70kDa Protein 1A (HSPA1A)

Buffer Formulation

0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.

Traits

Liquid

Concentration

500µg/mL

Organism Species More

Mus musculus (Mouse), Sus scrofa; Porcine (Pig)

Applications

WB,IHC

If the antibody is used in flow cytometry, please check FCM antibodies.

UOM

20µl 100µl 200µl 1ml 10ml

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against HSPA1A. It has been selected for its ability to recognize HSPA1A in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.5-3µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Magazine Citations
Journal of Neuroinflammation Identification of potential biomarkers of gold nanoparticle toxicity in rat brains PubMed: 22691312
Mediators Inflamm.? Local Inflammation in Fracture Hematoma: Results from a Combined Trauma Model in Pigs Pubmed:Pmc4324980
Eur J Pharmacol. Differential modulatory effects of morphine on acute and chronic stress induced neurobehavioral and cellular markers in rats Pubmed:24508522
Pharmacol Rep. Effect of propranolol on IL-10, visfatin, Hsp70, iNOS, TLR2, and survivin in amelioration of tumor progression and survival in Solid Ehrlich Carcinoma-bearing mice Pubmed:25443743
Advances in Medical Sciences A similar pro/anti-inflammatory cytokine balance is present in the airways of competitive athletes and non-exercising asthmatics pubmed:28822267
Archives of Medical Research Winter ambient training conditions are associated with increased bronchial hyperreactivity and with shifts in serum innate immunity proteins in young … Pubmed:29379533
metabolic brain disease Curcumin attenuates cytoplasmic/endoplasmic reticulum stress, apoptosis and cholinergic dysfunction in diabetic rat hippocampus Pubmed: 32172517
EXPERIMENTAL CELL RESEARCH Antagomirs targeting miR-142–5p attenuate pilocarpine-induced status epilepticus in mice Pubmed: 32439493
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