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SKU:MAA644Ra21

Monoclonal Antibody to CCAAT/Enhancer Binding Protein Alpha (CEBPa)

Monoclonal Antibody to CCAAT/Enhancer Binding Protein Alpha (CEBPa)

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UOM
Concentration
Organism species

Western blotting: 0.2-2µg/mL;1:500-5000
Immunohistochemistry: 5-20µg/mL;1:50-200
Immunocytochemistry: 5-20µg/mL;1:50-200
Optimal working dilutions must be determined by end user.

Product No.

MAA644Ra21

Organism Species

Rattus norvegicus (Rat).

Source

Monoclonal antibody preparation

Host

Mouse

Potency (Clone Number)

C1

Ig Isotype

IgG

Purification

Protein A + Protein G affinity chromatography

Label

None

Immunogen

RPA644Ra01-Recombinant CCAAT/Enhancer Binding Protein Alpha (CEBPa)

Buffer Formulation

0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.

Traits

Liquid

Concentration

1mg/mL

Organism Species More

Homo sapiens (Human)

Applications

WB; IHC; ICC; IP.

If the antibody is used in flow cytometry, please check FCM antibodies.

 UOM

20µl 100µl 200µl 1ml 10ml

SPECIFITY

The antibody is a mouse monoclonal antibody raised against CEBPa. It has been selected for its ability to recognize CEBPa in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Magazine Citations
Clin Cancer Res Inactivation of the p53–KLF4–CEBPA Axis in Acute Myeloid Leukemia PubMed: 26408402
Nutrients.  Hyperglycemia Changes Expression of Key Adipogenesis Markers (C/EBPα and PPARᵞ) and Morphology of Differentiating Human Visceral Adipocytes Pubmed: 31398873
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