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SKU:PAA591Mi01
Polyclonal Antibody to Proliferating Cell Nuclear Antigen (PCNA)
Polyclonal Antibody to Proliferating Cell Nuclear Antigen (PCNA)
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Western blotting: 0.5-2µg/mL;
;Immunohistochemistry: 5-20µg/mL;
;Immunocytochemistry: 5-20µg/mL;
;Optimal working dilutions must be determined by end user.
Product No.
PAA591Mi01
Organism Species
Homo sapiens (Human), Mus musculus (Mouse), Rattus norvegicus (Rat), Oryctolagus cuniculus (Rabbit), Rhesus monkey (Simian), Canis familiaris; Canine (Dog), Sus scrofa; Porcine (Pig).
Source
Polyclonal antibody preparation
Host
Rabbit
Potency
n/a
Ig Type
IgG
Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Label
None
Immunogen
RPA591Mi01-Recombinant Proliferating Cell Nuclear Antigen (PCNA)
Buffer Formulation
PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Traits
Liquid
Concentration
0.62mg/ml
Organism Species More
Mus musculus (Mouse), Rattus norvegicus (Rat), Cavia (Guinea pig ), Canis familiaris; Canine (Dog), Sus scrofa; Porcine (Pig), Bos taurus; Bovine (Cattle), Capra hircus; Caprine (Goat), Ovis aries; Ovine (Sheep), Equus caballus; Equine (Horse), Chicken (Gallus)
Applications
WB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
UOM
20µl 100µl 200µl 1ml 10ml
SPECIFITY
The antibody is a rabbit polyclonal antibody raised against PCNA. It has been selected for its ability to recognize PCNA in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.5-3µg/mL;
Immunohistochemistry: 5-30µg/mL;
Immunocytochemistry: 5-30µg/mL;
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
| Magazine | Citations |
| Tissue and Cell | Human umbilical cord blood-mesenchymal stem cells transplantation renovates the ovarian surface epithelium in a rat model of premature ovarian failure: Possible direct and indirect effects Pubmed:27233913 |
| Food and Chemical Toxicology | Antioxidant, anti-inflammatory and synergistic anti-hyperglycemic effects of Malaysian propolis and metformin in streptozotocin–induced diabetic rats Pubmed:30026088 |
| Life Sciences | Malaysian propolis, metformin and their combination, exert hepatoprotective effect in streptozotocin-induced diabetic rats Pubmed: 30205096 |
| Archives of Physiology and Biochemistry | Diabetes-induced testicular oxidative stress, inflammation, and caspase-dependent apoptosis: the protective role of metformin Pubmed: 30513216 |
| European journal of medicinal chemistry | Synthesis of novel S-linked dihydroartemisinin derivatives and evaluation of their anticancer activity Pubmed: 31216504 |
| Antioxidants (Basel). | The Effect of Dietary Quercetin on the Glutathione Redox System and Small Intestinal Functionality of Weaned Piglets Pubmed: 31426309 |
| Antioxidants | Oxidative Stress, NF-κB-Mediated Inflammation and Apoptosis in the Testes of Streptozotocin–Induced Diabetic Rats: Combined Protective Effects of Malaysian … Pubmed: 31600920 |
| TOXICOLOGY | Tert-butylhydroquinone preserve testicular steroidogenesis and spermatogenesis in cisplatin-intoxicated rats by targeting oxidative stress, inflammation and apoptosis Pubmed: 32565124 |
| BIOMEDICINE & PHARMACOTHERAPY | Protective effects of bee bread on testicular oxidative stress, NF-κB-mediated inflammation, apoptosis and lactate transport decline in obese male rats Pubmed: 33152939 |
| Sci Rep | Tert-butylhydroquinone attenuates doxorubicin-induced dysregulation of testicular cytoprotective and steroidogenic genes, and improves spermatogenesis in rats 33750916 |
| J Cell Sci | Local DNA synthesis is critical for DNA repair during oocyte maturation 34415018 |
| Stem Cell Rev Rep | Comparative Evaluation of Anti-Fibrotic Effect of Tissue Specific Mesenchymal Stem Cells Derived Extracellular Vesicles for the Amelioration of CCl4 Induced Chronic … 34859376 |
| Naunyn-Schmiedeberg's Archives | Inflammatory Modulation of miR-155 Inhibits Doxorubicin‑Induced Testicular Dysfunction Via Sirt1/Foxo1 Pathway: Insight to the Role of Acacetin and Bacillus … Pubmed:35715546 |
| Antioxidants | Therapeutic Effects of Bee Bread on Obesity-Induced Testicular-Derived Oxidative Stress, Inflammation, and Apoptosis in High-Fat Diet Obese Rat Model … Pubmed:35204140 |
| Non-POU Domain-Containing Octamer-Binding (NONO) Protein Stability Regulated by PIN1 is Crucial for Breast Cancer Tumorigenicity Via the MAPK/β … | |
| Impact de l'incorporation d'acides gras à chaine moyenne, de suppléments de levures et d'avoine nue sur la santé intestinale ainsi que sur la consommation … |
| Catalog No. | Related products for research use of Homo sapiens (Human), Mus musculus (Mouse), Rattus norvegicus (Rat), Oryctolagus cuniculus (Rabbit), Rhesus monkey (Simian), Canis familiaris; Canine (Dog), Sus scrofa; Porcine (Pig) Organism species | Applications (RESEARCH USE ONLY!) |
| RPA591Mi01 | Recombinant Proliferating Cell Nuclear Antigen (PCNA) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA591Mi01 | Polyclonal Antibody to Proliferating Cell Nuclear Antigen (PCNA) | WB; IHC; ICC; IP. |
| MAA591Mi22 | Monoclonal Antibody to Proliferating Cell Nuclear Antigen (PCNA) | WB; IHC; ICC; IP. |
| MAA591Mi23 | Monoclonal Antibody to Proliferating Cell Nuclear Antigen (PCNA) | WB; IHC; ICC; IP. |
| CAA591Mi22 | Anti-Proliferating Cell Nuclear Antigen (PCNA) Monoclonal Antibody | Loading Control of WB |
| MAA591Mi24 | Monoclonal Antibody to Proliferating Cell Nuclear Antigen (PCNA) | WB; IHC; ICC; IP. |
| MAA591Mi21 | Monoclonal Antibody to Proliferating Cell Nuclear Antigen (PCNA) | WB; IHC; ICC; IP. |
| SEA591Mi | ELISA Kit for Proliferating Cell Nuclear Antigen (PCNA) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA591Mi | Magnetic Luminex Assay Kit for Proliferating Cell Nuclear Antigen (PCNA) ,etc. | Magnetic Luminex Assay for Antigen Detection. |
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