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SKU:LAC038Ge71

Biotin-Linked Polyclonal Antibody to Diacylglycerol (DAG)

Biotin-Linked Polyclonal Antibody to Diacylglycerol (DAG)

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UOM
Concentration
Organism species

Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.

Product No.

LAC038Ge71

Organism Species

Pan-species (General).

Source

Antibody labeling

Ig Type

IgG

Purification

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Label

Biotin

Original Antibody

n/a

Buffer Formulation

PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.

Traits

Liquid

Concentration

500µg/mL

Organism Species More

n/a

Applications

WB; IHC; ICC.

If the antibody is used in flow cytometry, please check FCM antibodies.

UOM

20µl 100µl 200µl 1ml 10ml

 

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against DAG. It has been selected for its ability to recognize DAG in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

 

Magazine Citations
Acta Pharmacologica Sinica Wogonin ameliorates lipotoxicity-induced apoptosis of cultured vascular smooth muscle cells via interfering with DAG-PKC pathway Nature: 2011120a
Psychoneuroendocrinology Maternal stress predicts altered biogenesis and the profile of mitochondrial proteins in the frontal cortex and hippocampus of adult offspring rats PubMed: 26143539
The Journal of Biological Chemistry Aryl Hydrocarbon Receptor Plays Protective Roles against High Fat Diet (HFD)-induced Hepatic Steatosis and the Subsequent Lipotoxicity via Direct Transcriptional ReCavia (Guinea pig )lation of Socs3 Gene Expression Pubmed:26865635
Journal of the American Heart Association Pigment Epithelium-Derived Factor (PEDF) Improves Ischemic Cardiac Functional Reserve Through Decreasing Hypoxic Cardiomyocyte Contractility Through PEDF Receptor (PEDF-R). pubmed:27413044
International Journal of Molecular Medicine PEDF protects cardiomyocytes by promoting FUNDC1‑mediated mitophagy via PEDF-R under hypoxic condition Pubmed:29512692
British Journal of Pharmacology Bouchardatine analogue alleviates NAFLD/NASH in high fat fed mice via blunting ATP synthase activity Pubmed: 31113010
Gut Akkermansia muciniphila ameliorates non-alcoholic fatty liver disease by L-aspartate via interaction with liver
Histochem Cell Biol The expression of diacylglycerol kinase isoforms α and ζ correlates with the progression of experimental autoimmune encephalomyelitis in rats 34312706
Catalog No. Related products for research use of Pan-species (General) Organism species Applications (RESEARCH USE ONLY!)
CPC038Ge11 BSA Conjugated Diacylglycerol (DAG) Immunogen; SDS-PAGE; WB.
CPC038Ge21 OVA Conjugated Diacylglycerol (DAG) Immunogen; SDS-PAGE; WB.
PAC038Ge01 Polyclonal Antibody to Diacylglycerol (DAG) ELISA, CLIA. / IHC-Fr, ICC, IP (predicted).
LAC038Ge71 Biotin-Linked Polyclonal Antibody to Diacylglycerol (DAG) WB; IHC; ICC.
CEC038Ge ELISA Kit for Diacylglycerol (DAG) Enzyme-linked immunosorbent assay for Antigen Detection.
LMC038Ge Magnetic Luminex Assay Kit for Diacylglycerol (DAG) ,etc. Magnetic Luminex Assay for Antigen Detection.
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