Cloud-clone
SKU:LAL917Mu71
Biotin-Linked Polyclonal Antibody to Semaphorin 3A (SEMA3A)
Biotin-Linked Polyclonal Antibody to Semaphorin 3A (SEMA3A)
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Western blotting: 0.5-2ug/ml
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.
Product No.
LAL917Mu71
Organism Species
Mus musculus (Mouse).
Source
Antibody labeling
Ig Type
IgG
Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Label
Biotin
Original Antibody
PAL917Mu01-Polyclonal Antibody to Semaphorin 3A (SEMA3A)
Buffer Formulation
0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Traits
Liquid
Concentration
500µg/mL
Organism Species More
n/a
Applications
WB; IHC; ICC.
If the antibody is used in flow cytometry, please check FCM antibodies.
UOM
20µl 100µl 200µl 1ml 10ml
SPECIFITY
The antibody is a rabbit polyclonal antibody raised against SEMA3A. It has been selected for its ability to recognize SEMA3A in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.5-2ug/ml
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
| Magazine | Citations |
| Arthritis Research & Therapy | Semaphorin 3A is a marker for disease activity and a potential immunoregulator in systemic lupus erythematosus Biomed: ar3881 |
| journal of invastigative dermatology | Cathelicidin LL-37 Induces Semaphorin 3A Expression in Human Epidermal Keratinocytes: Implications for Possible Application to Pruritus PubMed: 26121211 |
| Neurology | Neurofilament light chain level is a weak risk factor for the development of MS. pubmed:27521440 |
| Development | miR-126-5p promotes retinal endothelial cell survival through SetD5 regulatio in neurons pubmed:29180574 |
| A Preliminary Study of the Effect of Semaphorin 3A and Acitretin on the Proliferation, Migration, and Apoptosis of HaCaT Cells | |
| Life Sciences | Curcumin and LOXblock-1 ameliorate ischemia-reperfusion induced inflammation and acute kidney injury by suppressing the semaphorin-plexin pathway. Pubmed: 32603817 |
| ACS NANO | Gold Nanoparticles Induce Tumor Vessel Normalization and Impair Metastasis by Inhibiting Endothelial Smad2/3 Signaling Pubmed: 32413258 |
| PLoS One | Extract of Scutellaria baicalensis induces semaphorin 3A production in human epidermal keratinocytes 33905439 |
| Catalog No. | Related products for research use of Mus musculus (Mouse) Organism species | Applications (RESEARCH USE ONLY!) |
| RPL917Mu01 | Recombinant Semaphorin 3A (SEMA3A) | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPL917Mu02 | Recombinant Semaphorin 3A (SEMA3A) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAL917Mu02 | Polyclonal Antibody to Semaphorin 3A (SEMA3A) | WB; IHC; ICC; IP. |
| PAL917Mu01 | Polyclonal Antibody to Semaphorin 3A (SEMA3A) | WB; IHC; ICC; IP. |
| LAL917Mu71 | Biotin-Linked Polyclonal Antibody to Semaphorin 3A (SEMA3A) | WB; IHC; ICC. |
| SEL917Mu | ELISA Kit for Semaphorin 3A (SEMA3A) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LML917Mu | Magnetic Luminex Assay Kit for Semaphorin 3A (SEMA3A) ,etc. | Magnetic Luminex Assay for Antigen Detection. |
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