Cloud-clone
SKU:LAA915Ge71
Biotin-Linked Polyclonal Antibody to 25-Hydroxyvitamin D3 (HVD3)
Biotin-Linked Polyclonal Antibody to 25-Hydroxyvitamin D3 (HVD3)
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Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.
Product No.
LAA915Ge71
Organism Species
Pan-species (General).
Source
Antibody labeling
Ig Type
IgG
Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Label
Biotin
Original Antibody
n/a
Buffer Formulation
PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Traits
Liquid
Concentration
500µg/mL
Organism Species More
n/a
Applications
WB; IHC; ICC.
If the antibody is used in flow cytometry, please check FCM antibodies.
UOM
20µl 100µl 200µl 1ml 10ml
SPECIFITY
The antibody is a rabbit polyclonal antibody raised against HVD3. It has been selected for its ability to recognize HVD3 in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
| Magazine | Citations |
| Allergy & Clinical Immunology | Decreased serum LL-37 and vitamin D3 levels in atopic dermatitis: relationship between IL-31 and oncostatin M Wiley: source |
| Tianjin Med J | 成人哮喘血清维生素D 水平与肺功能相关因素分析 Jwk_Tjyy: Cn |
| Journal of Functional Foods | Comparative effects of brown and golden flaxseeds on body composition, inflammation and bone remodelling biomarkers in perimenopausal overweight women S1756464617301561 |
| J Nutr Biochem. | Chronic maternal calcium and 25-hydroxyvitamin D deficiency in Wistar rats programs abnormal hepatic gene expression leading to hepatic steatosis in female offspring. pubmed:28219837 |
| in vivo | Effect of Calcitriol on FGF23 Level in Healthy Adults and its Dependence on Phosphate Level pubmed:28064234 |
| Asia pacific allergy | Serum 25-hydroxyvitamin D inversely associated with blood eosinophils in patients with persistent allergic rhinitis. pubmed:29094019 |
| Biomed Research International | Effects of Vitamin D Restricted Diet Administered during Perinatal and Postnatal Periods on the Penis of Wistar Rats Pubmed:29850540 |
| Clin Implant Dent Relat Res. | Evaluation of FGF‐23 and 25(OH)D3 levels in peri‐implant sulcus fluid in peri‐implant health and diseases Pubmed: 31407857 |
| JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY | Circulating Osteocyte‐Related Biomarkers (vitamin D, sclerostin, dickkopf-1), hepcidin, and oxidative stress markers in early breast cancer: their impact in … Pubmed: 33065276 |
| The Journal of Obstetrics and Gynecology of India | The Effects of Follicular Fluid 25 (OH) D Concentration on Intrafollicular Estradiol Level, Oocyte Quality, and Fertilization Rate in Women Who Underwent IVF Program |
| Catalog No. | Related products for research use of Pan-species (General) Organism species | Applications (RESEARCH USE ONLY!) |
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| PAA915Ge01 | Polyclonal Antibody to 25-Hydroxyvitamin D3 (HVD3) | WB; IHC; ICC; IP. |
| LAA915Ge71 | Biotin-Linked Polyclonal Antibody to 25-Hydroxyvitamin D3 (HVD3) | WB; IHC; ICC. |
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| CEA915Ge | ELISA Kit for 25-Hydroxyvitamin D3 (HVD3) | Enzyme-linked immunosorbent assay for Antigen Detection. |
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| KSA915Ge11 | ELISA Kit DIY Materials for 25-Hydroxyvitamin D3 (HVD3) | Main materials for "Do It (ELISA Kit) Yourself". |
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