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SKU:PAA239Mi08
Polyclonal Antibody to Alpha-Melanocyte Stimulating Hormone (aMSH)
Polyclonal Antibody to Alpha-Melanocyte Stimulating Hormone (aMSH)
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Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.
Product No.
PAA239Mi08
Organism Species
Homo sapiens (Human), Mus musculus (Mouse), Rattus norvegicus (Rat), Oryctolagus cuniculus (Rabbit), Sus scrofa; Porcine (Pig), Bos taurus; Bovine (Cattle), Ovis aries; Ovine (Sheep).
Source
Polyclonal antibody preparation
Host
Rabbit
Potency
n/a
Ig Type
IgG
Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Label
None
Immunogen
CPA239Mi21-OVA Conjugated Alpha-Melanocyte Stimulating Hormone (aMSH)
Buffer Formulation
PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Traits
Liquid
Concentration
500µg/mL
Organism Species More
n/a
Applications
WB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
UOM
20µl 100µl 200µl 1ml 10ml
SPECIFITY
The antibody is a rabbit polyclonal antibody raised against aMSH. It has been selected for its ability to recognize aMSH in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.5-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
| Magazine | Citations |
| J Biochem. | Generation of a human bone marrow-derived mesenchymal stem cell line expressing and secreting high levels of bioactive α-melanocyte-stimulating hormone. Pubmed: 23341471 |
| Pigment Cell Melanoma Res | Pharmacological induction of skin pigmentation unveils the neuroendocrine circuit regulated by light PubMed: 26582755 |
| The Journal of Biological Chemistry | Intermedin Restores Hyperhomocysteinemia-induced Macrophage Polarization and Improves Insulin Resistance in Mice Pubmed:27080257 |
| Pigment Cell & Melanoma Research | Pharmacological induction of skin pigmentation unveils the neuroendocrine circuit reCavia (Guinea pig )lated by light Pubmed:26582755 |
| Nature Communications | Endocrine disruptors induce perturbations in endoplasmic reticulum and mitochondria of human pluripotent stem cell derivatives. pubmed:28794470 |
| Cell Stem Cell | Super-Obese Patient-Derived iPSC Hypothalamic Neurons Exhibit Obesogenic Signatures and Hormone Responses Pubmed:29681516 |
| European Neuropsychopharmacology | Epigenetic alterations of the POMC promoter in tobacco dependence Pubmed:29871818 |
| Neuropeptides | Mechanisms of sustained long-term weight loss after RYGB: α-MSH is a key factor Pubmed:29685637 |
| Neuropsychobiology | Alcohol Withdrawal and Proopiomelanocortin Neuropeptides in an Animal Model of Alcohol Dependence Pubmed: 31117084 |
| INTERNATIONAL IMMUNOPHARMACOLOGY | Intermedin alleviates the inflammatory response and stabilizes the endothelial barrier in LPS-induced ARDS through the PI3K/Akt/eNOS signaling pathway Pubmed: 32892076 |
| J Endocrinol Invest | Serum alpha-melanocyte-stimulating hormone (a-MSH), brain-derived neurotrophic factor (BDNF), and agouti-related protein (AGRP) levels in children with Prader … Pubmed:35098494 |
| Catalog No. | Related products for research use of Homo sapiens (Human), Mus musculus (Mouse), Rattus norvegicus (Rat), Oryctolagus cuniculus (Rabbit), Sus scrofa; Porcine (Pig), Bos taurus; Bovine (Cattle), Ovis aries; Ovine (Sheep) Organism species | Applications (RESEARCH USE ONLY!) |
| CPA239Mi21 | OVA Conjugated Alpha-Melanocyte Stimulating Hormone (aMSH) | Immunogen; SDS-PAGE; WB. |
| SPA239Mi01 | Synthetic Alpha-Melanocyte Stimulating Hormone (aMSH) | Immunogen; Blocking Peptide. |
| PAA239Mi08 | Polyclonal Antibody to Alpha-Melanocyte Stimulating Hormone (aMSH) | WB; IHC; ICC; IP. |
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