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SKU:PAB486Hu08
Polyclonal Antibody to Calreticulin (CALR)
Polyclonal Antibody to Calreticulin (CALR)
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Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.
Product No.
PAB486Hu08
Organism Species
Homo sapiens (Human).
Source
Polyclonal antibody preparation
Host
Rabbit
Potency
n/a
Ig Type
IgG
Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Label
None
Immunogen
CPB486Hu21-OVA Conjugated Calreticulin (CALR)
Buffer Formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
Traits
Liquid
Concentration
500µg/mL
Organism Species More
n/a
Applications
WB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
UOM
20µl 100µl 200µl 1ml 10ml
SPECIFITY
The antibody is a rabbit polyclonal antibody raised against CALR. It has been selected for its ability to recognize CALR in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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Magazine |
Citations |
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Journal of Investigative Dermatology |
Oxidative Stress-Induced Calreticulin Expression and Translocation: New Insights into the Destruction of Melanocytes Pubmed: 23771121 |
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International Journal of Molecular Sciences |
The Anticoagulant Effect of PGI2S and tPA in Transgenic Umbilical Vein Endothelial Cells Is Linked to Up-Regulation of PKA and PKC Mdpi: Source |
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The Journal of Maternal-Fetal & Neonatal Medicine |
Amniotic fluid calreticulin in pregnancies complicated by the preterm prelabor rupture of membranes Pubmed:26953684 |
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journal of maternal-fetal & neonatal medicine |
Cervical fluid calreticulin and cathepsin-G in pregnancies complicated by preterm prelabor rupture of membranes. pubmed:28152632 |
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Talanta |
Investigation of the interaction between calreticulin and immunoglobulin G by capillary electrophoresis and computer modeling Doi: 10.1016/j.talanta.2018.11.098 |
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Sci Adv |
Thermostable ionizable lipid-like nanoparticle (iLAND) for RNAi treatment of hyperlipidemia Pubmed:35171673 |
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Catalog No. |
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