Cloud-clone
SKU:PAA950Hu02
Polyclonal Antibody to Von Willebrand Factor Cleaving Protease (vWFCP)
Polyclonal Antibody to Von Willebrand Factor Cleaving Protease (vWFCP)
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Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.
Product No.
PAA950Hu02
Organism Species
Homo sapiens (Human).
Source
Polyclonal antibody preparation
Host
Rabbit
Potency
n/a
Ig Type
IgG
Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Label
None
Immunogen
RPA950Hu02-Recombinant Von Willebrand Factor Cleaving Protease (vWFCP)
Buffer Formulation
0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Traits
Liquid
Concentration
1mg/mL
Organism Species More
n/a
Applications
WB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
UOM
20µl 100µl 200µl 1ml 10ml
SPECIFITY
The antibody is a rabbit polyclonal antibody raised against vWFCP. It has been selected for its ability to recognize vWFCP in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.5-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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Magazine |
Citations |
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33 |
Neutrophil Protease Cleavage of Von Willebrand Factor in Glomeruli - An Anti-thrombotic Mechanism in the Kidney. pubmed:28139439 |
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Experimental and Therapeutic Medicine |
Risk stratification and prognostic evaluation of endothelial cell‑specific molecule1, von Willebrand factor, and a disintegrin‑like and metalloprotease with … |
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Scientific Reports |
Association between Upper-airway Surgery and Ameliorative Risk Markers of endothelial function in obstructive Sleep Apnea Pubmed: 31882827 |
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Catalog No. |
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Applications (RESEARCH USE ONLY!) |
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RPA950Hu02 |
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PAA950Hu02 |
Polyclonal Antibody to Von Willebrand Factor Cleaving Protease (vWFCP) |
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Enzyme-linked immunosorbent assay for Antibody Detection. |
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