Cloud-clone
SKU:PAD057Ge01
Polyclonal Antibody to 7-Dehydrocholesterol (7-DHC)
Polyclonal Antibody to 7-Dehydrocholesterol (7-DHC)
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Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.
Product No.
PAD057Ge01
Organism Species
Pan-species (General).
Source
Polyclonal antibody preparation
Host
Rabbit
Potency
n/a
Ig Type
IgG
Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Label
None
Immunogen
CPD057Ge21-OVA Conjugated 7-Dehydrocholesterol (7-DHC)
Buffer Formulation
0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Traits
Liquid
Concentration
500µg/mL
Organism Species More
n/a
Applications
WB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
UOM
20µl 100µl 200µl 1ml 10ml
SPECIFITY
The antibody is a rabbit polyclonal antibody raised against 7-DHC. It has been selected for its ability to recognize 7-DHC in immunohistochemical staining and western blotting.
USAGE
Western blotting: 72.5ng/ml
Immunohistochemistry: 5-20µg/mL
Immunocytochemistry: 5-20µg/mL
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
| Catalog No. | Related products for research use of Pan-species (General) Organism species | Applications (RESEARCH USE ONLY!) |
| CPD057Ge21 | OVA Conjugated 7-Dehydrocholesterol (7-DHC) | Immunogen; SDS-PAGE; WB. |
| CPD057Ge11 | BSA Conjugated 7-Dehydrocholesterol (7-DHC) | Immunogen; SDS-PAGE; WB. |
| PAD057Ge01 | Polyclonal Antibody to 7-Dehydrocholesterol (7-DHC) | WB; IHC; ICC; IP. |
| CED057Ge | ELISA Kit for 7-Dehydrocholesterol (7-DHC) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMD057Ge | Magnetic Luminex Assay Kit for 7-Dehydrocholesterol (7-DHC) ,etc. | Magnetic Luminex Assay for Antigen Detection. |
| KSD057Ge11 | ELISA Kit DIY Materials for 7-Dehydrocholesterol (7-DHC) | Main materials for "Do It (ELISA Kit) Yourself". |
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