Cloud-clone
SKU:MAA835Hu21
Monoclonal Antibody to Corticotropin Releasing Factor (CRF)
Monoclonal Antibody to Corticotropin Releasing Factor (CRF)
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Western blotting: 0.5-5ug/ml
;Immunocytochemistry in formalin fixed cells: 5-30ug/ml
;Immunohistochemistry in formalin fixed frozen section: 5-30ug/ml
;Immunohistochemistry in paraffin section: 5-30ug/ml
;Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
;Optimal working dilutions must be determined by end user.
Product No.
MAA835Hu21
Organism Species
Homo sapiens (Human).
Source
Monoclonal antibody preparation
Host
Mouse
Potency (Clone Number)
E1
Ig Isotype
IgG1 Kappa
Purification
Protein A + Protein G affinity chromatography
Label
None
Immunogen
RPA835Hu04-Recombinant Corticotropin Releasing Factor (CRF)
Buffer Formulation
0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Traits
Liquid
Concentration
0.64mg/ml
Organism Species More
Rattus norvegicus (Rat)
Applications
WB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
UOM
20µl 100µl 200µl 1ml 10ml
SPECIFITY
The antibody is a mouse monoclonal antibody raised against CRF. It has been selected for its ability to recognize CRF in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.5-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
| Magazine | Citations |
| Toxicology Letters | Regulation of corticosterone secretion is modified by PFOS exposure at different levels of the hypothalamic–pituitary–adrenal axis in adult male rats Pubmed:24440345 |
| Bipolar?Disord. | Immune dysfunction in bipolar disorder and suicide risk: is there an association between peripheral corticotropin‐releasing hormone and interleukin‐1β? Pubmed:24862833 |
| Peptides | Differential stress response in rats subjected to chronic mild stress is accompanied by changes in CRH-family gene expression at the pituitary level Pubmed:25236411 |
| Ann Dermatol | Acute Stress-Induced Changes in Follicular Dermal Papilla Cells and Mobilization of Mast Cells:Implications for Hair Growth. pubmed:27746640 |
| Neuroscience | Heat stress-induced neuroinflammation and aberration in monoamine levels in hypothalamus are associated with temperature dysregulation pubmed:28663093 |
| Biomed Research International | Colonic Transit Time and Gut Peptides in Adult Patients with Slow and Normal Colonic Transit Constipation pubmed:29082242 |
| Biochemical Pharmacology | Effects of n-3 PUFA enriched and n-3 PUFA deficient diets in naïve and Aβ-treated female rats Pubmed:30028991 |
| Frontiers in Pharmacology | Vaccinium bracteatum Leaf Extract Reverses Chronic Restraint Stress-Induced Depression-Like Behavior in Mice: Regulation of Hypothalamic-Pituitary … Pubmed:30038568 |
| Toxicology Letters | Perfluorooctane sulfonate (PFOS) can alter the hypothalamic-pituitary-adrenal (HPA) axis activity by modifying CRF1 and glucocorticoid receptors Pubmed:29807116 |
| Neuropsychiatric disease and treatment | The depressive-like behaviors of chronic unpredictable mild stress-treated mice, ameliorated by Tibetan medicine Zuotai: involvement in the hypothalamic … Pubmed:29379286 |
| BMC Veterinary Research | Hypothalamus-pituitary-adrenal axis involves in anti-viral ability through regulation of immune response in piglets infected by highly pathogenic porcine … Pubmed:29540178 |
| Bioscience Research | Bioscience Research |
| FASEB Journal | Contribution of DNA methylation in chronic stress–induced cardiac remodeling and arrhythmias in mice Pubmed: 31431066 |
| Aging Cell | Altered glucocorticoid metabolism represents a feature of macroph‐aging Pubmed: 32463582 |
| Endocr J | Impairment of CRH in the intestinal mucosal epithelial barrier of pregnant Bama miniature pig induced by restraint stress 33408312 |
| CRYOBIOLOGY | O-GlcNAc/Akt pathway regulates glucose metabolism and reduces apoptosis in liver of piglets with acute cold stress 33651993 |
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