Cloud-clone
SKU:LAA234Hu72
Biotin-Linked Monoclonal Antibody to Immunoglobulin G4 (IgG4)
Biotin-Linked Monoclonal Antibody to Immunoglobulin G4 (IgG4)
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Western blotting: 0.2-2µg/mL;1:500-5000
Immunohistochemistry: 5-20µg/mL;1:50-200
Immunocytochemistry: 5-20µg/mL;1:50-200
Optimal working dilutions must be determined by end user.
Product No.
LAA234Hu72
Organism Species
Homo sapiens (Human).
Source
Antibody labeling
Ig Isotype
IgG
Purification
Protein A + Protein G affinity chromatography
Label
Biotin
Original Antibody
n/a
Buffer Formulation
PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Traits
Liquid
Concentration
1mg/mL
Organism Species More
n/a
Applications
WB; IHC; ICC.
If the antibody is used in flow cytometry, please check FCM antibodies.
UOM
20µl 100µl 200µl 1ml 10ml
SPECIFITY
The antibody is a mouse monoclonal antibody raised against IgG4. It has been selected for its ability to recognize IgG4 in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.2-2µg/mL;1:500-5000
Immunohistochemistry: 5-20µg/mL;1:50-200
Immunocytochemistry: 5-20µg/mL;1:50-200
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
| Magazine | Citations |
| BioMed Research International | The Effect of CXC Motif Chemokine 13 on Hepatocellular Carcinoma Associates with Wnt Signaling Hindawi:Source |
| Adv Med Sci. | Utility of serum IgG, IgG4 and carbonic anhydrase II antibodies in distinguishing autoimmune pancreatitis from pancreatic cancer and chronic pancreatitis Pubmed:25194335 |
| Oncology Reports | Beneficial effect of T follicular helper cells on antibody class switching of B cells in prostate cancer Pubmed:25529861 |
| Iranian Journal of Pharmaceutical Research | Effect of Cysteamine on Cell Growth and IgG4 Production in Recombinant Sp2.0 Cells Pubmed:25561924 |
| Ann Hematol | Loss of interleukin-21 leads to atrophic germinal centers in multicentric Castleman’s disease PubMed: 26377996 |
| Biomed Res Int | The Effect of C-X-C Motif Chemokine 13 on Hepatocellular Carcinoma Associates with Wnt Signaling PubMed: 26161394 |
| 网页进不去，NCBI上搜不到 | Increasing Foxp3, IgG4 and improvement quality of life after immunotherapy treatment in allergic rhinitis patients article:30785 |
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