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SKU:LAJ322Ra74

Biotin-Linked Monoclonal Antibody to Cystatin B (CSTB)

Biotin-Linked Monoclonal Antibody to Cystatin B (CSTB)

Regular price $330.00 USD
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UOM
Concentration
Organism species

Western blotting: 0.5-2ug/ml
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.

Product No.

LAJ322Ra74

Organism Species

Rattus norvegicus (Rat).

Source

Antibody labeling

Ig Isotype

IgG

Purification

Protein A + Protein G affinity chromatography

Label

Biotin

Original Antibody

MAJ322Ra26-Monoclonal Antibody to Cystatin B (CSTB)

Buffer Formulation

0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.

Traits

Liquid

Concentration

1mg/mL

Organism Species More

n/a

Applications

WB; IHC; ICC.

If the antibody is used in flow cytometry, please check FCM antibodies.

UOM

20µl 100µl 200µl 1ml 10ml

SPECIFITY

The antibody is a mouse monoclonal antibody raised against CSTB. It has been selected for its ability to recognize CSTB in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.5-2ug/ml
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Magazine Citations
AIDS. Cathepsin B and cystatin B in HIV-seropositive women are associated with infection and HIV-1-associated neurocognitive disorders PubMed: 23291538
International Journal of Circumpolar Health Evaluation of serum procathepsin B, cystatin B and cystatin C as possible biomarkers of ovarian cancer PubMed: PMC3754495
PLoS One. Dysregulation of Macrophage-Secreted Cathepsin B Contributes to HIV-1-Linked Neuronal Apoptosis PubMed: PMC3365072
Journal of Neuroimmune Pharmacology Cocaine potentiates cathepsin B secretion and neuronal apoptosis from HIV-infected macrophages Pubmed:25209871
European Journal of Cancer Supplements A91: Endogenous inhibitors of cysteine proteases and preform of cathepsin B in cancer of reproductive system Science: Article
Annals of Clinical Biochemistry Diagnostic specificity and sensitivity of PIVKAII, GP3, CSTB, SCCA1 and HGF for the diagnosis of hepatocellular carcinoma in patients with alcoholic liver cirrhosis. pubmed:28766361
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