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SKU:LAA635Ra76

Biotin-Linked Monoclonal Antibody to Complement Factor H (CFH)

Biotin-Linked Monoclonal Antibody to Complement Factor H (CFH)

Regular price $398.00 USD
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UOM
Concentration
Organism species

Western blotting: 0.5-2ug/ml
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.

Product No.

LAA635Ra76

Organism Species

Rattus norvegicus (Rat).

Source

Antibody labeling

Ig Isotype

IgG

Purification

Protein A + Protein G affinity chromatography

Label

Biotin

Original Antibody

MAA635Ra26-Monoclonal Antibody to Complement Factor H (CFH)

Buffer Formulation

0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.

Traits

Liquid

Concentration

1mg/mL

Organism Species More

n/a

Applications

WB; IHC; ICC.

If the antibody is used in flow cytometry, please check FCM antibodies.

UOM

20µl 100µl 200µl 1ml 10ml

SPECIFITY

The antibody is a mouse monoclonal antibody raised against CFH. It has been selected for its ability to recognize CFH in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.5-2ug/ml
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Magazine Citations
Pediatr Int Atypical hemolytic uremic syndrome: Korean pediatric series PubMed: 25443527
Hernia Circulating matrix metalloproteinases and procollagen propeptides in inguinal hernia Pubmed:29484522
Mikrochim Acta Fluorescent silver nanoclusters as antibody label in a competitive immunoassay for the complement factor H Pubmed: 31187271
Diagnostics Systemic Alterations of Immune Response-Related Proteins during Glaucoma Development in the Murine Model DBA/2J Pubmed: 32585848
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE The importance of sialic acid, pH and ion concentration on the interaction of uromodulin and complement factor H 33788378
Front Immunol A Family Affair: Addressing the Challenges of Factor H and the Related Proteins 33868311
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