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SKU:MAE676Hu26

Monoclonal Antibody to Regenerating Islet Derived Protein 3 Gamma (REG3g)

Monoclonal Antibody to Regenerating Islet Derived Protein 3 Gamma (REG3g)

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UOM
Concentration
Organism species

Western blotting: 0.5-3µg/mL;
;Immunohistochemistry: 5-30µg/mL;
;Immunocytochemistry: 5-30µg/mL;
;Optimal working dilutions must be determined by end user.

Product No.

MAE676Hu26

Organism Species

Homo sapiens (Human).

Source

Monoclonal antibody preparation

Host

Mouse

Potency (Clone Number)

D9

Ig Isotype

IgG2a Kappa

Purification

Protein A + Protein G affinity chromatography

Label

None

Immunogen

RPE676Hu01-Recombinant Regenerating Islet Derived Protein 3 Gamma (REG3g)

Buffer Formulation

0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.

Traits

Liquid

Concentration

1mg/mL

Organism Species More

Rattus norvegicus (Rat), Sus scrofa; Porcine (Pig)

Applications

WB

If the antibody is used in flow cytometry, please check FCM antibodies.

UOM

20µl 100µl 200µl 1ml 10ml

SPECIFITY

The antibody is a mouse monoclonal antibody raised against REG3g. It has been selected for its ability to recognize REG3g in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.2-3µg/mL;
Immunohistochemistry: 5-30µg/mL;
Immunocytochemistry: 5-30µg/mL;
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Magazine Citations
16 Innate Stat3-mediated induction of the antimicrobial protein Reg3γ is required for host defense against MRSA pneumonia PubMed: PMC3600913
Biology of Blood and Marrow Transplatation Reciprocal Expression of Enteric Antimicrobial Proteins in Intestinal Graft-Versus-Host Disease Pubmed: 23927965
Nature Comunication Hyperglycaemia inhibits REG3A expression to exacerbate TLR3-mediated skin inflammation in diabetes. pubmed:27830702
Gastroenterology Enteric Delivery of Regenerating Family Member 3 alpha Alters the Intestinal Microbiota and Controls Inflammation in Mice With Colitis Pubmed:29133078
Kidney International Interleukin-6/Stat3 signaling has an essential role in the host antimicrobial response to urinary tract infection Pubmed:29475562
Journal of Immunology Research Role of TLR5 in the Translocation and Dissemination of Commensal Bacteria in the Intestine after Traumatic Hemorrhagic Shock 34840990
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