Cloud-clone
SKU:LAB226Ra76
Biotin-Linked Monoclonal Antibody to Corticosteroid Binding Globulin (CBG)
Biotin-Linked Monoclonal Antibody to Corticosteroid Binding Globulin (CBG)
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Western blotting: 0.5-2ug/ml
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.
Product No.
LAB226Ra76
Organism Species
Rattus norvegicus (Rat).
Source
Antibody labeling
Ig Isotype
IgG
Purification
Protein A + Protein G affinity chromatography
Label
Biotin
Original Antibody
MAB226Ra26-Monoclonal Antibody to Corticosteroid Binding Globulin (CBG)
Buffer Formulation
0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Traits
Liquid
Concentration
1mg/mL
Organism Species More
n/a
Applications
WB; IHC; ICC.
If the antibody is used in flow cytometry, please check FCM antibodies.
UOM
20µl 100µl 200µl 1ml 10ml
SPECIFITY
The antibody is a mouse monoclonal antibody raised against CBG. It has been selected for its ability to recognize CBG in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.5-2ug/ml
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
| Magazine | Citations |
| General Endocrinology | The Regulation of Rasd1 Expression by Glucocorticoids and Prolactin Controls Peripartum Maternal Insulin Secretion Endjournals: Source |
| PLoS One | Modulation in Wistar Rats of Blood Corticosterone Compartmentation by Sex and a Cafeteria Diet PubMed: PMC3579843 |
| Annals of Anatomy | Differences in colocalization of corticosteroid-binding globulin and glucocorticoid receptor immunoreactivity in the rat brain ScienceDirect: S0940960212001628 |
| Physiological Reports | Adrenocortical control in the neonatal rat: ACTH‐and cAMP‐independent corticosterone production during hypoxia Pubmed: 24303136 |
| Shock. | Adrenocorticotropic Hormone But Not High-Density Lipoprotein Cholesterol or Salivary Cortisol Was a Predictor of Adrenal Insufficiency in Patients With Septic Shock. Pubmed: 24667620 |
| Neurobiology of Aging | Greater glucocorticoid receptor activation in hippocampus of aged rats sensitizes microglia Pubmed:25559333 |
| Cell Death Differ. | Novel p53 target genes secreted by the liver are involved in non-cell-autonomous reCavia (Guinea pig )lation Pubmed:26358154 |
| Journal of Exercise, Sports & Orthopedics | Impact of Season and Oral Contraceptive use on Cortisol Levelsin Physically Active Women exercise-sports-orthopedics:exercise-sports-orthop |
| Impact of Season and Oral Contraceptive use on Cortisol Levels in Physically Active Women diva2:1047030 | |
| Neuropharmacology | Impaired glucocorticoid-mediated HPA axis negative feedback induced by juvenile social isolation in male rats Pubmed:29407214 |
| Pharmacological Reports | The impact of early-life stress on corticosteroid carrier protein levels and 11β-hydroxysteroid dehydrogenase 1 expression in adolescent rats |
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