Cloud-clone
SKU:LAC141Hu82
FITC-Linked Monoclonal Antibody to Collagen Type IV Alpha 5 (COL4a5)
FITC-Linked Monoclonal Antibody to Collagen Type IV Alpha 5 (COL4a5)
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Western blotting: 0.5-2ug/ml
;Immunocytochemistry in formalin fixed cells: 5-20ug/ml
;Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
;Immunohistochemistry in paraffin section: 5-20ug/ml
;Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
;Optimal working dilutions must be determined by end user.
Product No.
LAC141Hu82
Organism Species
Homo sapiens (Human).
Source
Antibody labeling
Ig Isotype
IgG
Purification
Protein A + Protein G affinity chromatography
Label
FITC
Original Antibody
MAC141Hu21-Monoclonal Antibody to Collagen Type IV Alpha 5 (COL4a5)
Buffer Formulation
0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Traits
Liquid
Concentration
1mg/mL
Organism Species More
n/a
Applications
WB; IHC; ICC; IF.
If the antibody is used in flow cytometry, please check FCM antibodies.
UOM
20µl 100µl 200µl 1ml 10ml
SPECIFITY
The antibody is a mouse monoclonal antibody raised against COL4a5. It has been selected for its ability to recognize COL4a5 in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.5-2ug/ml
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
| Magazine | Citations |
| Experimental and Molecular Pathology | Epitope recognized by anti-glomerular basement membrane (GBM) antibody in a patient with repeated relapse of anti-GBM disease Pubmed: 30817909 |
| American Journal of Respiratory Cell and Molecular Biology | Basement Membrane Remodelling Controls Endothelial Function in IPAH Pubmed: 32160015 |
| Catalog No. | Related products for research use of Homo sapiens (Human) Organism species | Applications (RESEARCH USE ONLY!) |
| RPC141Hu01 | Recombinant Collagen Type IV Alpha 5 (COL4a5) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAC141Hu01 | Polyclonal Antibody to Collagen Type IV Alpha 5 (COL4a5) | WB |
| LAC141Hu71 | Biotin-Linked Polyclonal Antibody to Collagen Type IV Alpha 5 (COL4a5) | WB; IHC; ICC. |
| MAC141Hu21 | Monoclonal Antibody to Collagen Type IV Alpha 5 (COL4a5) | WB; IHC; ICC; IP. |
| MAC141Hu22 | Monoclonal Antibody to Collagen Type IV Alpha 5 (COL4a5) | WB; IHC; ICC; IP. |
| LAC141Hu82 | FITC-Linked Monoclonal Antibody to Collagen Type IV Alpha 5 (COL4a5) | WB; IHC; ICC; IF. |
| SEC141Hu | ELISA Kit for Collagen Type IV Alpha 5 (COL4a5) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMC141Hu | Magnetic Luminex Assay Kit for Collagen Type IV Alpha 5 (COL4a5) ,etc. | Magnetic Luminex Assay for Antigen Detection. |
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