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SKU:LAC585Hu72

Biotin-Linked Monoclonal Antibody to Urocortin 2 (UCN2)

Biotin-Linked Monoclonal Antibody to Urocortin 2 (UCN2)

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UOM
Concentration
Organism species

Western blotting: 0.5-2ug/ml
;Immunocytochemistry in formalin fixed cells: 5-20ug/ml
;Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
;Immunohistochemistry in paraffin section: 5-20ug/ml
;Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
;Optimal working dilutions must be determined by end user.

Product No.

LAC585Hu72

Organism Species

Homo sapiens (Human).

Source

Antibody labeling

Ig Isotype

IgG

Purification

Protein A + Protein G affinity chromatography

Label

Biotin

Original Antibody

MAC585Hu22-Monoclonal Antibody to Urocortin 2 (UCN2)

Buffer Formulation

0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.

Traits

Liquid

Concentration

1mg/mL

Organism Species More

n/a

Applications

WB; IHC; ICC.

If the antibody is used in flow cytometry, please check FCM antibodies.

UOM

20µl 100µl 200µl 1ml 10ml

SPECIFITY

The antibody is a mouse monoclonal antibody raised against UCN2. It has been selected for its ability to recognize UCN2 in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.5-2ug/ml
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Magazine Citations
Clin Chem. High-Sensitivity Sandwich ELISA for Plasma NT-proUcn2: Plasma Concentrations and Relationship to Mortality in Heart Failure. Pubmed:27127183
Clinica Chimica Acta Urocortins: Actions in health and heart failure pubmed:28887029
Cardiovascular Research Urocortin-2 improves right ventricular function and attenuates pulmonary arterial hypertension Pubmed:29584808
Journal of Neuroendocrinology Glucocorticoid withdrawal affects stress‐induced changes in urocortin 2 gene expression in the rat adrenal medulla and brain Pubmed:29604138
High Blood Press Cardiovasc Prev Evaluation of Serum Urocortin 2 Levels in Patients with Hypertension Pubmed: 31925709
CURRENT PROBLEMS IN CARDIOLOGY Urocortin-2 in acute heart failure: role as a marker of volume overload and pulmonary hypertension 33994037
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