Cloud-clone
SKU:LAA041Ra72
Biotin-Linked Monoclonal Antibody to Chemokine (C-X-C Motif) Ligand 1 (CXCL1)
Biotin-Linked Monoclonal Antibody to Chemokine (C-X-C Motif) Ligand 1 (CXCL1)
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Western blotting: 0.5-2ug/ml
;Immunocytochemistry in formalin fixed cells: 5-20ug/ml
;Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
;Immunohistochemistry in paraffin section: 5-20ug/ml
;Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
;Optimal working dilutions must be determined by end user.
Product No.
LAA041Ra72
Organism Species
Rattus norvegicus (Rat).
Source
Antibody labeling
Ig Isotype
IgG
Purification
Protein A + Protein G affinity chromatography
Label
Biotin
Original Antibody
MAA041Ra21-Monoclonal Antibody to Chemokine (C-X-C Motif) Ligand 1 (CXCL1)
Buffer Formulation
0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Traits
Liquid
Concentration
1mg/mL
Organism Species More
n/a
Applications
WB; IHC; ICC.
If the antibody is used in flow cytometry, please check FCM antibodies.
UOM
20µl 100µl 200µl 1ml 10ml
SPECIFITY
The antibody is a mouse monoclonal antibody raised against CXCL1. It has been selected for its ability to recognize CXCL1 in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.5-2ug/ml
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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| Phytotherapy Research | Total flavonoids from sea buckthorn ameliorates lipopolysaccharide/cigarette smoke‐induced airway inflammation Pubmed: 31209984 |
| Frontiers in Oncology | CXCL1 as an Unfavorable Prognosis Factor Negatively Regulated by DACH1 in Non-small Cell Lung Cancer Pubmed: 31998654 |
| Phytomedicine | Baohuoside I suppresses breast cancer metastasis by downregulating the tumor-associated macrophages/CXC motif chemokine ligand 1 pathway Pubmed: 32911383 |
| Lab Invest | Extracellular cold-inducible RNA-binding protein regulates neutrophil extracellular trap formation and tissue damage in acute pancreatitis Pubmed: 32709888 |
| Phytomedicine | Aiduqing formula suppresses breast cancer metastasis via inhibiting CXCL1-mediated autophagy |
| Cell Commun Signal | Aiduqing formula inhibits breast cancer metastasis by suppressing TAM/CXCL1-induced Treg differentiation and infiltration 34461944 |
| Viruses | Interleukin-10-Mediated Lymphopenia Caused by Acute Infection with Foot-and-Mouth Disease Virus in Mice 34960627 |
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