Cloud-clone
SKU:LAB851Hu71
Biotin-Linked Polyclonal Antibody to Vascular Endothelial Growth Factor 121 (VEGF121)
Biotin-Linked Polyclonal Antibody to Vascular Endothelial Growth Factor 121 (VEGF121)
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Western blotting: 0.5-2µg/mL
;Immunocytochemistry in formalin fixed cells: 5-20µg/mL
;Immunohistochemistry in formalin fixed frozen section: 5-20µg/mL
;Immunohistochemistry in paraffin section: 5-20µg/mL
;Optimal working dilutions must be determined by end user.
Product No.
LAB851Hu71
Organism Species
Homo sapiens (Human).
Source
Antibody labeling
Ig Type
IgG
Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Label
Biotin
Original Antibody
PAB851Hu01-Polyclonal Antibody to Vascular Endothelial Growth Factor 121 (VEGF121)
Buffer Formulation
0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Traits
Liquid
Concentration
500µg/mL
Organism Species More
n/a
Applications
WB; IHC; ICC.
If the antibody is used in flow cytometry, please check FCM antibodies.
UOM
20µl 100µl 200µl 1ml 10ml
SPECIFITY
The antibody is a rabbit polyclonal antibody raised against VEGF121. It has been selected for its ability to recognize VEGF121 in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.5-2µg/mL
Immunocytochemistry in formalin fixed cells: 5-20µg/mL
Immunohistochemistry in formalin fixed frozen section: 5-20µg/mL
Immunohistochemistry in paraffin section: 5-20µg/mL
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
| Magazine | Citations |
| Oncogene | Elevated PLGF contributes to small-cell lung cancer brain metastasis PubMed: 22797069 |
| BMC Cancer | VEGF-121 plasma level as biomarker for response to anti-angiogenetic therapy in recurrent glioblastoma Pubmed:29747600 |
| JOURNAL OF CLINICAL NEUROSCIENCE | A pediatric case of anaplastic astrocytoma with a gliomatosis cerebri; the growth pattern and changes in serum VEGF-121 levels after bevacizumab treatment Pubmed: 33222957 |
| INTERNATIONAL JOURNAL OF DEVELOPMENTAL NEUROSCIENCE | Associations between VEGF isoforms and impending retinopathy of prematurity Pubmed: 32737903 |
| international journal of oncology | Activin A triggers angiogenesis via regulation of VEGFA and its overexpression is associated with poor prognosis of oral squamous cell carcinoma Pubmed: 32377747 |
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