Cloud-clone
SKU:MAB870Hu21
Monoclonal Antibody to Tubulin Beta (TUBb)
Monoclonal Antibody to Tubulin Beta (TUBb)
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Western blotting: 0.5-2µg/mL;1:500-2000
;Immunohistochemistry: 5-20µg/mL;1:50-200
;Immunocytochemistry: 5-20µg/mL;1:50-200
;Optimal working dilutions must be determined by end user.
Product No.
MAB870Hu21
Organism Species
Homo sapiens (Human).
Source
Monoclonal antibody preparation
Host
Mouse
Potency (Clone Number)
C6
Ig Isotype
IgG2b Kappa
Purification
Protein A + Protein G affinity chromatography
Label
None
Immunogen
RPB870Hu01-Recombinant Tubulin Beta (TUBb)
Buffer Formulation
PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Traits
Liquid
Concentration
1mg/ml
Organism Species More
Cavia (Guinea pig ), Bos taurus; Bovine (Cattle), Capra hircus; Caprine (Goat), Chicken (Gallus)
Applications
WB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
UOM
20µl 100µl 200µl 1ml 10ml
SPECIFITY
The antibody is a mouse monoclonal antibody raised against TUBb. It has been selected for its ability to recognize TUBb in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-40µg/mL;
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
| Magazine | Citations |
| archives of pharmacal research | Synthesis and cytotoxic activity of certain trisubstituted azetidin-2-one derivatives as a cis-restricted combretastatin A-4 analogues pubmed:27747473 |
| archiv der pharmazie (Weinheim). | New 3-Substituted-2-(4-hydroxyanilino)pyridine Derivatives: Synthesis, Antitumor Activity, and Tubulin Polymerization Inhibition. pubmed:28150327 |
| ACS Applied Materials & Interfaces | Novel Beta-Tubulin-Immobilized Nanoparticles Affinity Material for Screening β-Tubulin Inhibitors from a Complex Mixture pubmed:28112513 |
| Future Medicinal Chemistry | Design, synthesis and biological evaluation of chromenopyrimidines as potential cytotoxic agents Pubmed:29779400 |
| Future Medicinal Chemistry | Design and synthesis of novel 5-(4-chlorophenyl) furan derivatives with inhibitory activity on tubulin polymerization Pubmed:29966433 |
| Journal of Biological Chemistry | Biochemical characterization of the PHARC associated serine hydrolase ABHD12 reveals its preference for very long chain lipids Pubmed: 30237167 |
| Journal of Enzyme Inhibition and Medicinal Chemistry | Design, synthesis, and screening of amino thiophene carboxamide derivatives on hepatocellular carcinomaas VEGFR-2Inhibitors Pubmed: 30191744 |
| European Journal of Medicinal Chemistry | Potent combretastatin A-4 analogs containing 1, 2, 4-triazole: Synthesis, antiproliferative, anti-tubulin activity, and docking study Pubmed: 31536891 |
| ACS Sensors | Early diagnosis of Alzheimer's disease in blood using a disposable electrochemical microfluidic platform Pubmed: 32207606 |
| Bioorganic Chemistry | Tubulin Inhibitors: Discovery of a New Scaffold Targeting Extra-binding Residues within the Colchicine Site through Anchoring Substituents Properly Adapted to their … Pubmed: 32325332 |
| BRITISH JOURNAL OF NUTRITION | Ginger and turmeric lipid-solubles attenuate heated oil-induced cardio-hepatic oxidative stress via the upregulation of NRF2 and decrease blood pressure in rats Pubmed: 33028437 |
| European Journal of Medicinal Chemistry | Design, Synthesis and Screening of Benzimidazole Containing Compounds with Methoxylated Aryl Radicals as Cytotoxic Molecules on (HCT-116) Colon … Pubmed: 33158579 |
| J BIOMOL STRUCT DYN/JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS | Cytotoxicity, in silico predictions and molecular studies for androstane heterocycle compounds revealed potential antitumor agent against lung cancer cells 33300466 |
| Cancer Cell International | Bufalin Induced Mitochondrial Dysfunction Promotes Apoptosis of Glioma Cells by Regulating Annexin A2 and DRP1 Proteins |
| Cancer Cell Int | Bufalin induces mitochondrial dysfunction and promotes apoptosis of glioma cells by regulating Annexin A2 and DRP1 protein expression 34376212 |
| Sci Rep | Curcumin derivative ST09 modulates the miR-199a-5p/DDR1 axis and regulates proliferation and migration in ovarian cancer cells 34837026 |
| Catalog No. | Related products for research use of Homo sapiens (Human) Organism species | Applications (RESEARCH USE ONLY!) |
| APB870Hu01 | Active Tubulin Beta (TUBb) | Cell culture; Activity Assays. |
| RPB870Hu01 | Recombinant Tubulin Beta (TUBb) | Positive Control; Immunogen; SDS-PAGE; WB. |
| CAB870Hu01 | Anti-Tubulin Beta (TUBb) Polyclonal Antibody | Loading Control of WB. |
| PAB870Hu01 | Polyclonal Antibody to Tubulin Beta (TUBb) | WB; IHC; ICC/IF |
| MAB870Hu22 | Monoclonal Antibody to Tubulin Beta (TUBb) | WB; IHC; ICC; IP. |
| MAB870Hu23 | Monoclonal Antibody to Tubulin Beta (TUBb) | WB; IHC; ICC; IP. |
| MAB870Hu25 | Monoclonal Antibody to Tubulin Beta (TUBb) | WB; IHC; ICC; IP. |
| MAB870Hu27 | Monoclonal Antibody to Tubulin Beta (TUBb) | WB; IHC; ICC; IP. |
| MAB870Hu24 | Monoclonal Antibody to Tubulin Beta (TUBb) | WB; IHC; ICC; IP. |
| MAB870Hu21 | Monoclonal Antibody to Tubulin Beta (TUBb) | WB; IHC; ICC; IP. |
| CAB870Hu22 | Anti-Tubulin Beta (TUBb) Monoclonal Antibody | Loading Control of WB. |
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