Cloud-clone
SKU:MAD117Hu21
Monoclonal Antibody to T-Cell Surface Glycoprotein CD3 Epsilon (CD3e)
Monoclonal Antibody to T-Cell Surface Glycoprotein CD3 Epsilon (CD3e)
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Western blotting: 0.5-5µg/mL
;Immunohistochemistry: 5-30µg/mL
;Immunocytochemistry: 5-30µg/mL
;Optimal working dilutions must be determined by end user.
Product No.
MAD117Hu21
Organism Species
Homo sapiens (Human).
Source
Monoclonal antibody preparation
Host
Mouse
Potency (Clone Number)
C10
Ig Isotype
IgG1 Kappa
Purification
Protein A + Protein G affinity chromatography
Label
None
Immunogen
RPD117Hu01-Recombinant T-Cell Surface Glycoprotein CD3 Epsilon (CD3e)
Buffer Formulation
PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Traits
Liquid
Concentration
1mg/ml
Organism Species More
n/a
Applications
WB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
UOM
20µl 100µl 200µl 1ml 10ml
SPECIFITY
The antibody is a mouse monoclonal antibody raised against CD3e. It has been selected for its ability to recognize CD3e in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.01-3µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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