Cloud-clone
SKU:RAA777Hu21
Recombinant Antibody to Inducible T-Cell Co Stimulator (ICOS)
Recombinant Antibody to Inducible T-Cell Co Stimulator (ICOS)
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Western blotting: 1:40-1:400
;Immunohistochemistry: 1:40-1:200
;Immunocytochemistry: 1:40-1:200
;Flow cytometry: 0.2-2µL/106 cells
;Optimal working dilutions must be determined by end user.
Product No.
RAA777Hu21
Organism Species
Homo sapiens (Human).
Source
Recombinant monoclonal antibody preparation
Host
Mouse
Potency (Clone Number)
n/a
Ig Isotype
IgG
Purification
Protein A + Protein G affinity chromatography
Label
None
Immunogen
RPA777Hu01-Recombinant Inducible T-Cell Co Stimulator (ICOS)
Buffer Formulation
0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Traits
Liquid
Concentration
0.1-1mg/ml
Organism Species More
n/a
Applications
WB; IFC; IHC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
UOM
20µl 100µl 200µl 1ml 10ml
SPECIFITY
The antibody is a mouse monoclonal antibody raised against ICOS. It has been selected for its ability to recognize ICOS in immunohistochemical staining and western blotting.
USAGE
Western blotting: 1:40-1:400
Immunohistochemistry: 1:40-1:200
Immunocytochemistry: 1:40-1:200
Flow cytometry: 0.2-2µL/106 cells
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
| Magazine | Citations |
| Archives of Dermatological Research | Increased production of soluble inducible costimulator in patients with diffuse cutaneous systemic sclerosis Pubmed: 23053221 |
| DNA Cell Biol. | Effect of soluble inducible costimulator level and its polymorphisms on age-related macular degeneration. Pubmed: 24083358 |
| Rheumatology | Augmented ICOS expression in patients with early diffuse cutaneous systemic sclerosis Pubmed: 23024058 |
| Br J Haematol | Inducible T‐cell co‐stimulator (ICOS) and ICOS ligand are novel players in the multiple‐myeloma microenvironment Pubmed:34954822 |
| Diagnostics (Basel) | Increased Levels of ICOS and ICOSL Are Associated to Pulmonary Arterial Hypertension in Patients Affected by Connective Tissue Diseases Pubmed:35328257 |
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