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SKU:PAF557Mi08
Polyclonal Antibody to Uncoupling Protein 1, Mitochondrial (UCP1)
Polyclonal Antibody to Uncoupling Protein 1, Mitochondrial (UCP1)
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Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.
Product No.
PAF557Mi08
Organism Species.
Source
Polyclonal antibody preparation
Host
Rabbit
Potency
n/a
Ig Type
IgG
Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Label
None
Immunogen
n/a
Buffer Formulation
PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Traits
Liquid
Concentration
500µg/mL
Organism Species More
n/a
Applications
WB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
UOM
20µl 100µl 200µl 1ml 10ml
SPECIFITY
The antibody is a rabbit polyclonal antibody raised against UCP1. It has been selected for its ability to recognize UCP1 in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
| Magazine | Citations |
| Stem Cells | FM19G11 Favors Spinal Cord Injury Regeneration and Stem Cell Self-Renewal by Mitochondrial Uncoupling and Glucose Metabolism Induction PubMed: 22865656 |
| Diabetes. | Inorganic nitrate promotes the browning of white adipose tissue through the nitrate-nitrite-nitric oxide pathway Pubmed:25249574 |
| Journal of Cachexia, Sarcopenia and Muscle | Muscle wasting and adipose tissue browning in infantile nephropathic cystinosis Doi: 10.1002 |
| FASEB J | Single-cell transcriptomics and functional target validation of brown adipocytes show their complex roles in metabolic homeostasis PubMed: 26304220 |
| FASEB JOURNAL | Irradiation of carotid baroreceptor with low‐intensity pulsed ultrasound exerts different metabolic protection in perirenal, epididymal white adipose tissue and … Pubmed: 32954574 |
| Brown and beige adipose tissue regulate systemic metabolism to resist diet-induced obesity through metabolite signals in an inter-organ signaling axis | |
| Science reports | All-trans-retinoic acid ameliorates atherosclerosis, promotes perivascular adipose tissue browning, and increases adiponectin production in Apo-E mice 33627760 |
| Nature Communications | Brown and beige adipose tissue regulate systemic metabolism through a metabolite interorgan signaling axis 33772024 |
| Catalog No. | Related products for research use of Organism species | Applications (RESEARCH USE ONLY!) |
| PAF557Mi08 | Polyclonal Antibody to Uncoupling Protein 1, Mitochondrial (UCP1) | WB; IHC; ICC; IP. |
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