Cloud-clone
SKU:MAB340Hu21
Monoclonal Antibody to Beta Actin (ACTB)
Monoclonal Antibody to Beta Actin (ACTB)
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Western blotting: 1/10000-1/10000000 (0.1ng/ml-100ng/ml)
;Immunohistochemistry: 5-20µg/mL;
;Immunocytochemistry: 5-20µg/mL;
;Optimal working dilutions must be determined by end user.
Product No.
MAB340Hu21
Organism Species
Homo sapiens (Human).
Source
Monoclonal antibody preparation
Host
Mouse
Potency (Clone Number)
C2
Ig Isotype
IgG2b Kappa
Purification
Protein A + Protein G affinity chromatography
Label
None
Immunogen
RPB340Hu01-Recombinant Beta Actin (ACTB)
Buffer Formulation
PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Traits
Liquid
Concentration
1mg/ml
Organism Species More
n/a
Applications
WB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
UOM
20µl 100µl 200µl 1ml 10ml
SPECIFITY
The antibody is a mouse monoclonal antibody raised against ACTB. It has been selected for its ability to recognize ACTB in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.01-3µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-30µg/mL;
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
| Magazine | Citations |
| International Journal of Molecular Sciences | Nano-Nutrition of Chicken Embryos. The Effect of in Ovo Administration of Diamond Nanoparticles and L-Glutamine on Molecular Responses in Chicken Embryo Pectoral Muscles NCBI:PMC3856104 |
| European Journal of BioMedical Research | Expression of chloride channels in mouse skin incisional wounds 96 |
| Cell Metabolism | Targeted Elimination of Senescent Beta Cells Prevents Type 1 Diabetes |
| Biological & Pharmaceutical Bulletin | Neuroinflammation, Oxidative Stress, and Neurogenesis in a Mouse Model of Chronic Fatigue Syndrome, and the Treatment with Kampo Medicine Pubmed: 31902915 |
| Biological research | Linc-OIP5 in the breast cancer cells regulates angiogenesis of human umbilical vein endothelial cells through YAP1/Notch/NRP1 signaling circuit at a tumor … Pubmed: 32046779 |
| JOURNAL OF INVESTIGATIVE MEDICINE | Effect of ischemic postconditioning on cell apoptosis and expression of relevant genes in non-culprit coronary arteries Pubmed: 32784207 |
| bioscience biotechnology research asia | Malted Barley Improved the Structure and Function of Gastrocnemius Muscle of Hypercholesterolemic Mother Rats and their Offspring |
| Genes | Tryptophan Depletion Modulates Tryptophanyl-tRNA Synthetase-Mediated High-Affinity Tryptophan Uptake into Human Cells 33261077 |
| Nat Commun | POLRMT mutations impair mitochondrial transcription causing neurological disease 33602924 |
| Life Sci | Indole-linked 1, 2, 3-triazole derivatives efficiently modulate COX-2 protein levels in human THP-1 monocytes by suppressing AGE-ROS-NF-kβ nexus Pubmed:34990649 |
| J Ethnopharmacol | Isolation and characterization of anti-inflammatory and anti-proliferative compound, for B-cell Non-Hodgkin lymphoma, from Nyctanthes arbor-tristis Linn. Pubmed:35398498 |
| Multimedia Tools and Applications | Investigation of single beam ultrasound sensitivity as a monitoring tool for local hyperthermia treatment in breast cancer |
| Catalog No. | Related products for research use of Homo sapiens (Human) Organism species | Applications (RESEARCH USE ONLY!) |
| RPB340Hu01 | Recombinant Beta Actin (ACTB) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB340Hu01 | Polyclonal Antibody to Beta Actin (ACTB) | WB; IHC; ICC; IP. |
| CAB340Hu01 | Anti-Beta Actin (ACTB) Polyclonal Antibody | Loading Control of WB. |
| MAB340Hu22 | Monoclonal Antibody to Beta Actin (ACTB) | WB; IHC; ICC; IP. |
| MAB340Hu23 | Monoclonal Antibody to Beta Actin (ACTB) | WB; IHC; ICC; IP. |
| MAB340Hu24 | Monoclonal Antibody to Beta Actin (ACTB) | WB; IHC; ICC; IP. |
| MAB340Hu21 | Monoclonal Antibody to Beta Actin (ACTB) | WB; IHC; ICC; IP. |
| MAB340Hu25 | Monoclonal Antibody to Beta Actin (ACTB) | WB; IHC; ICC; IP. |
| CAB340Hu22 | Anti-Beta Actin (ACTB) Monoclonal Antibody | Loading Control of WB |
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