Cloud-clone
SKU:PAD116Hu02
Polyclonal Antibody to Hedgehog Homolog, Indian (IHH)
Polyclonal Antibody to Hedgehog Homolog, Indian (IHH)
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Western blotting: 0.5-2µg/mL;1:500-2000
;Immunohistochemistry: 5-20µg/mL;1:50-200
;Immunocytochemistry: 5-20µg/mL;1:50-200
;Optimal working dilutions must be determined by end user.
Product No.
PAD116Hu02
Organism Species
Homo sapiens (Human).
Source
Polyclonal antibody preparation
Host
Rabbit
Potency
n/a
Ig Type
IgG
Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Label
None
Immunogen
RPD116Hu02-Recombinant Hedgehog Homolog, Indian (IHH)
Buffer Formulation
PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Traits
Liquid
Concentration
1mg/ml
Organism Species More
Mus musculus (Mouse)
Applications
WB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
UOM
20µl 100µl 200µl 1ml 10ml
SPECIFITY
The antibody is a rabbit polyclonal antibody raised against IHH. It has been selected for its ability to recognize IHH in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.5-2µg/mL;1:500-2000
Immunohistochemistry: 5-20µg/mL;1:50-200
Immunocytochemistry: 5-20µg/mL;1:50-200
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
| Magazine | Citations |
| The Journal of Biological Chemistry | Prostate cancer cells and bone stromal cells mutually interact with each other through bone morphogenetic protein-mediated signals PubMed: 22532569 |
| Cancer Letters | Defective CFTR promotes intestinal proliferation via inhibition of the hedgehog pathway during cystic fibrosis Pubmed: 30639531 |
| Journal of Hepatology | Eugenia Marbach-Breitrück, Madlen Matz-Soja, Ute Abraham, Wolfgang Schmidt-Heck, Susanne Sales, Christiane Rennert, Matthias Kern, Susanne Aleithe, Luise?… Pubmed: 30711403 |
| Tick-Tock hedgehog–mutual crosstalk with liver circadian clock renders hepatic hedgehog signaling a risk factor of steatosis | |
| Molecular Medicine Reports | MicroRNA-1 promotes cartilage matrix synthesis and regulates chondrocyte differentiation via post-transcriptional suppression of Ihh expression Pubmed: 32705199 |
| Catalog No. | Related products for research use of Homo sapiens (Human) Organism species | Applications (RESEARCH USE ONLY!) |
| APD116Hu01 | Active Hedgehog Homolog, Indian (IHH) | Cell culture; Activity Assays. |
| RPD116Hu02 | Recombinant Hedgehog Homolog, Indian (IHH) | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPD116Hu01 | Recombinant Hedgehog Homolog, Indian (IHH) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAD116Hu02 | Polyclonal Antibody to Hedgehog Homolog, Indian (IHH) | WB; IHC; ICC; IP. |
| PAD116Hu01 | Polyclonal Antibody to Hedgehog Homolog, Indian (IHH) | WB; IHC; ICC; IP. |
| LAD116Hu71 | Biotin-Linked Polyclonal Antibody to Hedgehog Homolog, Indian (IHH) | WB; IHC; ICC. |
| MAD116Hu21 | Monoclonal Antibody to Hedgehog Homolog, Indian (IHH) | WB; IHC; ICC; IP. |
| SED116Hu | ELISA Kit for Hedgehog Homolog, Indian (IHH) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMD116Hu | Magnetic Luminex Assay Kit for Hedgehog Homolog, Indian (IHH) ,etc. | Magnetic Luminex Assay for Antigen Detection. |
| KSD116Hu01 | ELISA Kit DIY Materials for Hedgehog Homolog, Indian (IHH) | Main materials for "Do It (ELISA Kit) Yourself". |
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