Cloud-clone
SKU:MAC343Mu21
Monoclonal Antibody to Heat Shock 70kDa Protein 5 (HSPA5)
Monoclonal Antibody to Heat Shock 70kDa Protein 5 (HSPA5)
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Western blotting: 0.5-2µg/mL;1:500-2000
;Immunohistochemistry: 5-20µg/mL;1:50-200
;Immunocytochemistry: 5-20µg/mL;1:50-200
;Optimal working dilutions must be determined by end user.
Product No.
MAC343Mu21
Organism Species
Mus musculus (Mouse).
Source
Monoclonal antibody preparation
Host
Mouse
Potency (Clone Number)
C5
Ig Isotype
IgG2b Kappa
Purification
Protein A + Protein G affinity chromatography
Label
None
Immunogen
RPC343Mu01-Recombinant Heat Shock 70kDa Protein 5 (HSPA5)
Buffer Formulation
0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Traits
Liquid
Concentration
1mg/ml
Organism Species More
Homo sapiens (Human), Mus musculus (Mouse), Rattus norvegicus (Rat)
Applications
WB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
UOM
20µl 100µl 200µl 1ml 10ml
SPECIFITY
The antibody is a mouse monoclonal antibody raised against HSPA5. It has been selected for its ability to recognize HSPA5 in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.5-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
| Magazine | Citations |
| 41 | Deficiency of Rac1 Blocks NADPH Oxidase Activation, Inhibits Endoplasmic Reticulum Stress, and Reduces Myocardial Remodeling in a Mouse Model of Type 1 Diabetes. PubMed: 20522592 |
| pharmacological research | Neuroprotection in non-transgenic and transgenic mouse models of Alzheimer's disease by positive modulation of σ1 receptors Pubmed: 31048034 |
| EPILEPSY & BEHAVIOR | Fenfluramine acts as a positive modulator of sigma-1 receptors Pubmed: 32169824 |
| Journal of Oncology | Using Serological Proteome Analysis to Identify and Evaluate Anti-GRP78 Autoantibody as Biomarker in the Detection of Gastric Cancer 33381181 |
| Heliyon | Glucose-regulated protein 78 (GRP78) in renal cell carcinoma: A novel biomarker for predicting tumor behavior |
| Medicine (Baltimore) | Levels of circulating GRP78 and CHOP in endoplasmic reticulum stress pathways in Chinese type 2 diabetic kidney disease patients 34414940 |
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