Cloud-clone
SKU:MAB342Hu21
Monoclonal Antibody to Actin Alpha 2, Smooth Muscle (ACTa2)
Monoclonal Antibody to Actin Alpha 2, Smooth Muscle (ACTa2)
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Western blotting: 0.5-2µg/mL;
;Immunohistochemistry: 5-20µg/mL;
;Immunocytochemistry: 5-20µg/mL;
;Optimal working dilutions must be determined by end user.
Product No.
MAB342Hu21
Organism Species
Homo sapiens (Human).
Source
Monoclonal antibody preparation
Host
Mouse
Potency (Clone Number)
E102
Ig Isotype
IgG1 Kappa
Purification
Protein A + Protein G affinity chromatography
Label
None
Immunogen
RPB342Hu01-Recombinant Actin Alpha 2, Smooth Muscle (ACTa2)
Buffer Formulation
PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Traits
Liquid
Concentration
1mg/ml
Organism Species More
n/a
Applications
WB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
UOM
20µl 100µl 200µl 1ml 10ml
SPECIFITY
The antibody is a mouse monoclonal antibody raised against ACTa2. It has been selected for its ability to recognize ACTa2 in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
| Magazine | Citations |
| Cell Mol Immunol | Toxoplasma gondii GRA15II effector-induced M1 cells ameliorate liver fibrosis in mice infected with Schistosomiasis japonica Pubmed:27157496 |
| NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY | Effects of repeated increasing doses of cisplatin as models of acute kidney injury and chronic kidney disease in rats Pubmed: 32936352 |
| Brazilian Journal of Pharmaceutical Sciences | Hesperidin inhibits the epithelial to mesenchymal transition induced by transforming growth factor-β1 in A549 cells through Smad signaling in the cytoplasm |
| Structural Changes and Detection of Liver Fibrosis¨CRelated Protein Levels in Coculture of Alveolar Echinococcosis-Protoscoleces and Human Hepatic Stellate cells | |
| Parasit Vectors | Structural changes and expression of hepatic fibrosis-related proteins in coculture of Echinococcus multilocularis protoscoleces and human hepatic stellate … 34857049 |
| Clinical and Translational Medicine | NOX5 mediates the crosstalk between tumor cells and cancer‐associated fibroblasts via regulating cytokine network 34459125 |
| Catalog No. | Related products for research use of Homo sapiens (Human) Organism species | Applications (RESEARCH USE ONLY!) |
| RPB342Hu01 | Recombinant Actin Alpha 2, Smooth Muscle (ACTa2) | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPB342Hu02 | Recombinant Actin Alpha 2, Smooth Muscle (ACTa2) | Positive Control; Immunogen; SDS-PAGE; WB. |
| CPB342Hu21 | OVA Conjugated Actin Alpha 2, Smooth Muscle (ACTa2) | Immunogen; SDS-PAGE; WB. |
| PAB342Hu01 | Polyclonal Antibody to Actin Alpha 2, Smooth Muscle (ACTa2) | WB; IHC; ICC; IP. |
| CAB342Hu01 | Anti-Actin Alpha 2, Smooth Muscle (ACTa2) Polyclonal Antibody | Loading Control of WB. |
| MAB342Hu22 | Monoclonal Antibody to Actin Alpha 2, Smooth Muscle (ACTa2) | WB; IHC; ICC; IP. |
| MAB342Hu21 | Monoclonal Antibody to Actin Alpha 2, Smooth Muscle (ACTa2) | WB; IHC; ICC; IP. |
| CAB342Hu22 | Anti-Actin Alpha 2, Smooth Muscle (ACTa2) Monoclonal Antibody | Loading Control of WB |
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