Cloud-clone
SKU:MAA591Mi21
Monoclonal Antibody to Proliferating Cell Nuclear Antigen (PCNA)
Monoclonal Antibody to Proliferating Cell Nuclear Antigen (PCNA)
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Western blotting: 0.5-2µg/mL;
;Immunohistochemistry: 5-20µg/mL;
;Immunocytochemistry: 5-20µg/mL;
;Optimal working dilutions must be determined by end user.
Product No.
MAA591Mi21
Organism Species
Homo sapiens (Human), Mus musculus (Mouse), Rattus norvegicus (Rat), Canis familiaris; Canine (Dog), Sus scrofa; Porcine (Pig).
Source
Monoclonal antibody preparation
Host
Mouse
Potency (Clone Number)
C4
Ig Isotype
IgG2b Kappa
Purification
Protein A + Protein G affinity chromatography
Label
None
Immunogen
RPA591Mi01-Recombinant Proliferating Cell Nuclear Antigen (PCNA)
Buffer Formulation
0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Traits
Liquid
Concentration
1mg/ml
Organism Species More
Cavia (Guinea pig ), Bos taurus; Bovine (Cattle), Capra hircus; Caprine (Goat), Ovis aries; Ovine (Sheep)
Applications
WB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
UOM
20µl 100µl 200µl 1ml 10ml
SPECIFITY
The antibody is a mouse monoclonal antibody raised against PCNA. It has been selected for its ability to recognize PCNA in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
| Magazine | Citations |
| Tissue and Cell | Human umbilical cord blood-mesenchymal stem cells transplantation renovates the ovarian surface epithelium in a rat model of premature ovarian failure: Possible direct and indirect effects Pubmed:27233913 |
| Food and Chemical Toxicology | Antioxidant, anti-inflammatory and synergistic anti-hyperglycemic effects of Malaysian propolis and metformin in streptozotocin–induced diabetic rats Pubmed:30026088 |
| Life Sciences | Malaysian propolis, metformin and their combination, exert hepatoprotective effect in streptozotocin-induced diabetic rats Pubmed: 30205096 |
| Archives of Physiology and Biochemistry | Diabetes-induced testicular oxidative stress, inflammation, and caspase-dependent apoptosis: the protective role of metformin Pubmed: 30513216 |
| European journal of medicinal chemistry | Synthesis of novel S-linked dihydroartemisinin derivatives and evaluation of their anticancer activity Pubmed: 31216504 |
| Antioxidants (Basel). | The Effect of Dietary Quercetin on the Glutathione Redox System and Small Intestinal Functionality of Weaned Piglets Pubmed: 31426309 |
| Antioxidants | Oxidative Stress, NF-κB-Mediated Inflammation and Apoptosis in the Testes of Streptozotocin–Induced Diabetic Rats: Combined Protective Effects of Malaysian … Pubmed: 31600920 |
| TOXICOLOGY | Tert-butylhydroquinone preserve testicular steroidogenesis and spermatogenesis in cisplatin-intoxicated rats by targeting oxidative stress, inflammation and apoptosis Pubmed: 32565124 |
| BIOMEDICINE & PHARMACOTHERAPY | Protective effects of bee bread on testicular oxidative stress, NF-κB-mediated inflammation, apoptosis and lactate transport decline in obese male rats Pubmed: 33152939 |
| Sci Rep | Tert-butylhydroquinone attenuates doxorubicin-induced dysregulation of testicular cytoprotective and steroidogenic genes, and improves spermatogenesis in rats 33750916 |
| J Cell Sci | Local DNA synthesis is critical for DNA repair during oocyte maturation 34415018 |
| Stem Cell Rev Rep | Comparative Evaluation of Anti-Fibrotic Effect of Tissue Specific Mesenchymal Stem Cells Derived Extracellular Vesicles for the Amelioration of CCl4 Induced Chronic … 34859376 |
| Naunyn-Schmiedeberg's Archives | Inflammatory Modulation of miR-155 Inhibits Doxorubicin‑Induced Testicular Dysfunction Via Sirt1/Foxo1 Pathway: Insight to the Role of Acacetin and Bacillus … Pubmed:35715546 |
| Antioxidants | Therapeutic Effects of Bee Bread on Obesity-Induced Testicular-Derived Oxidative Stress, Inflammation, and Apoptosis in High-Fat Diet Obese Rat Model … Pubmed:35204140 |
| Non-POU Domain-Containing Octamer-Binding (NONO) Protein Stability Regulated by PIN1 is Crucial for Breast Cancer Tumorigenicity Via the MAPK/β … | |
| Impact de l'incorporation d'acides gras à chaine moyenne, de suppléments de levures et d'avoine nue sur la santé intestinale ainsi que sur la consommation … |
| Catalog No. | Related products for research use of Homo sapiens (Human), Mus musculus (Mouse), Rattus norvegicus (Rat), Canis familiaris; Canine (Dog), Sus scrofa; Porcine (Pig) Organism species | Applications (RESEARCH USE ONLY!) |
| RPA591Mi01 | Recombinant Proliferating Cell Nuclear Antigen (PCNA) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA591Mi01 | Polyclonal Antibody to Proliferating Cell Nuclear Antigen (PCNA) | WB; IHC; ICC; IP. |
| MAA591Mi22 | Monoclonal Antibody to Proliferating Cell Nuclear Antigen (PCNA) | WB; IHC; ICC; IP. |
| MAA591Mi23 | Monoclonal Antibody to Proliferating Cell Nuclear Antigen (PCNA) | WB; IHC; ICC; IP. |
| CAA591Mi22 | Anti-Proliferating Cell Nuclear Antigen (PCNA) Monoclonal Antibody | Loading Control of WB |
| MAA591Mi24 | Monoclonal Antibody to Proliferating Cell Nuclear Antigen (PCNA) | WB; IHC; ICC; IP. |
| MAA591Mi21 | Monoclonal Antibody to Proliferating Cell Nuclear Antigen (PCNA) | WB; IHC; ICC; IP. |
| SEA591Mi | ELISA Kit for Proliferating Cell Nuclear Antigen (PCNA) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA591Mi | Magnetic Luminex Assay Kit for Proliferating Cell Nuclear Antigen (PCNA) ,etc. | Magnetic Luminex Assay for Antigen Detection. |
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