Cloud-clone
SKU:CEA634Mu
ELISA Kit for Trypsinogen Activation Peptide (TAP)
ELISA Kit for Trypsinogen Activation Peptide (TAP)
Couldn't load pickup availability
Enzyme-linked immunosorbent assay for Antigen Detection.
Product No.
CEA634Mu
Organism Species
Mus musculus (Mouse).
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Test Method
Competitive Inhibition
Assay Length
2h
Detection Range
123.5-10,000pg/mL
Sensitivity
The minimum detectable dose of this kit is typically less than 53.5pg/mL.
UOM
48T 96T 96T*5 96T*10 96T*100
Specificity
This assay has high sensitivity and excellent specificity for detection of Trypsinogen Activation Peptide (TAP).
No significant cross-reactivity or interference between Trypsinogen Activation Peptide (TAP) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Trypsinogen Activation Peptide (TAP) and the recovery rates were calculated by comparing the measured value to the expected amount of Trypsinogen Activation Peptide (TAP) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 90-101 | 95 |
| EDTA plasma(n=5) | 87-101 | 92 |
| heparin plasma(n=5) | 80-91 | 83 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Trypsinogen Activation Peptide (TAP) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Trypsinogen Activation Peptide (TAP) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Trypsinogen Activation Peptide (TAP) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 81-90% | 81-95% | 82-89% | 84-97% |
| EDTA plasma(n=5) | 92-105% | 99-105% | 82-91% | 80-104% |
| heparin plasma(n=5) | 78-101% | 91-98% | 96-103% | 91-104% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
| Standard | 2 | Standard Diluent | 1×20mL |
| Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
| Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
| TMB Substrate | 1×9mL | Stop Solution | 1×6mL |
| Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.
| Magazine | Citations |
| Diabetologia | Diabetes aggravates acute pancreatitis and inhibits pancreas regeneration in mice SpringerLink: x4l01435t34452jr |
| Molecular Biology Reports | Prior peritoneal lavage with hot 0.9% saline induces HSP70 expression and protects against cerulein-induced acute pancreatitis in rats Pubmed: 23096089 |
| Journal of the Pancreas | Case report of mannose-binding lectin (MBL) deficiency and postoperative sepsis and coagulopathy in a patient following total pancreatectomy for chronic pancreatitis. Pubmed:25262717 |
| Journal of Gastroenterology | A small molecule inhibitor of NFκB blocks ER stress and the NLRP3 inflammasome and prevents progression of pancreatitis pubmed:27418337 |
| Indian Journal of Pharmaceutical Sciences | Effects of Calcium Channel Blockers on Trypsinogen Activation and Severity of Cerulein-induced Acute Pancreatitis in Rats IJPS:Source |
| Tropical Journal of Pharmaceutical Research | Investigation of non-pharmaceutical cures for acute pancreatitis induced by cerulein in rats |
| Pancreas | The Regulatory Effect of the Kinase Inhibitor PD98059 on Autophagic Flux During Trypsinogen Activation in Pancreatic Acinar Cells Pubmed: 32011537 |
| Catalog No. | Related products for research use of Mus musculus (Mouse) Organism species | Applications (RESEARCH USE ONLY!) |
| CPA634Mu11 | BSA Conjugated Trypsinogen Activation Peptide (TAP) | Immunogen; SDS-PAGE; WB. |
| CPA634Mu21 | OVA Conjugated Trypsinogen Activation Peptide (TAP) | Immunogen; SDS-PAGE; WB. |
| CEA634Mu | ELISA Kit for Trypsinogen Activation Peptide (TAP) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA634Mu | Magnetic Luminex Assay Kit for Trypsinogen Activation Peptide (TAP) ,etc. | Magnetic Luminex Assay for Antigen Detection. |
| KSA634Mu11 | ELISA Kit DIY Materials for Trypsinogen Activation Peptide (TAP) | Main materials for "Do It (ELISA Kit) Yourself". |
Share
