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ELISA Kit for Meningioma Expressed Antigen 5 (MGEA5)
ELISA Kit for Meningioma Expressed Antigen 5 (MGEA5)
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Enzyme-linked immunosorbent assay for Antigen Detection.
Product No.
SEA069Hu
Organism Species
Homo sapiens (Human).
Sample Type
Serum, plasma, tissue homogenates and other biological fluids
Test Method
Double-antibody Sandwich
Assay Length
3h
Detection Range
1.56-100ng/mL
Sensitivity
The minimum detectable dose of this kit is typically less than 0.56ng/mL.
UOM
48T 96T 96T*5 96T*10 96T*100
Specificity
This assay has high sensitivity and excellent specificity for detection of Meningioma Expressed Antigen 5 (MGEA5).
No significant cross-reactivity or interference between Meningioma Expressed Antigen 5 (MGEA5) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Meningioma Expressed Antigen 5 (MGEA5) and the recovery rates were calculated by comparing the measured value to the expected amount of Meningioma Expressed Antigen 5 (MGEA5) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 79-97 | 85 |
| EDTA plasma(n=5) | 78-96 | 88 |
| heparin plasma(n=5) | 88-102 | 92 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Meningioma Expressed Antigen 5 (MGEA5) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Meningioma Expressed Antigen 5 (MGEA5) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Meningioma Expressed Antigen 5 (MGEA5) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 93-101% | 78-104% | 97-105% | 94-102% |
| EDTA plasma(n=5) | 89-96% | 83-97% | 79-95% | 83-91% |
| heparin plasma(n=5) | 91-102% | 88-101% | 78-93% | 78-94% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
| Standard | 2 | Standard Diluent | 1×20mL |
| Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
| Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
| TMB Substrate | 1×9mL | Stop Solution | 1×6mL |
| Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
| Magazine | Citations |
| Plos one | Metal-on-Metal Hip Prostheses and Systemic Health: A Cross-Sectional Association Study 8 Years after Implantation PubMed: PMC3677913 |
| Renal Failure | Evaluation of the Process of Recycling and Renal Parenchymal Injury after ESWL with Metabolites Excreted in the Urine Pubmed: 23413820 |
| BioMed Research International | The Renal Effects of Vanadate Exposure: Potential Biomarkers and Oxidative Stress as a Mechanism of Functional Renal Disorders—Preliminary Studies Hindawi: 740105 |
| PLoS One | Ameliorative Effect of Chrysin on Adenine-Induced Chronic Kidney Disease in Rats PubMed: 25909514 |
| Ecotoxicol Environ Saf | Melamine and oxalate coexposure induces early kidney tubular injury through mitochondrial aberrations and oxidative stress 34507040 |
| Antioxidants | Diminishment of Nrf2 Antioxidative Defense Aggravates Nephrotoxicity of Melamine and Oxalate Coexposure. Antioxidants 2021, 10, 1464 34573096 |
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