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SKU:SEB021Mu
ELISA Kit for Beta Catenin (β-catenin)
ELISA Kit for Beta Catenin (β-catenin)
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Enzyme-linked immunosorbent assay for Antigen Detection.
Product No.
SEB021Mu
Organism Species
Mus musculus (Mouse).
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Test Method
Double-antibody Sandwich
Assay Length
3h
Detection Range
0.156-10ng/mL
Sensitivity
The minimum detectable dose of this kit is typically less than 0.060ng/mL.
UOM
48T 96T 96T*5 96T*10 96T*100
Specificity
This assay has high sensitivity and excellent specificity for detection of Beta Catenin (β-catenin).
No significant cross-reactivity or interference between Beta Catenin (β-catenin) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Beta Catenin (β-catenin) and the recovery rates were calculated by comparing the measured value to the expected amount of Beta Catenin (β-catenin) in samples.
|
Matrix |
Recovery range (%) |
Average(%) |
|
serum(n=5) |
94-102 |
97 |
|
EDTA plasma(n=5) |
82-96 |
90 |
|
heparin plasma(n=5) |
92-101 |
98 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Beta Catenin (β-catenin) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Beta Catenin (β-catenin) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Beta Catenin (β-catenin) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
1:16 |
|
serum(n=5) |
88-97% |
90-104% |
90-101% |
94-105% |
|
EDTA plasma(n=5) |
88-97% |
96-104% |
78-94% |
84-101% |
|
heparin plasma(n=5) |
79-103% |
83-105% |
80-96% |
87-102% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|
Reagents |
Quantity |
Reagents |
Quantity |
|
Pre-coated, ready to use 96-well strip plate |
1 |
Plate sealer for 96 wells |
4 |
|
Standard |
2 |
Standard Diluent |
1×20mL |
|
Detection Reagent A |
1×120µL |
Assay Diluent A |
1×12mL |
|
Detection Reagent B |
1×120µL |
Assay Diluent B |
1×12mL |
|
TMB Substrate |
1×9mL |
Stop Solution |
1×6mL |
|
Wash Buffer (30 × concentrate) |
1×20mL |
Instruction manual |
1 |
Assay procedure summary
-
Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
|
Magazine |
Citations |
|
Clinica Chimica Acta |
Versican and its associated molecules: Potential diagnostic markers for multiple myeloma Pubmed:25623955 |
|
Journal of Bone and Mineral Research |
Adverse effects of osteocytic constitutive activation of ?-catenin on bone strength and bone growth Pubmed:25639729 |
|
Am J Transl Res |
Role of SDF-1 and Wnt signaling pathway in the myocardial fibrosis of hypertensive rats PubMed: 26396666 |
|
Scientific Reports |
Is there an association between enhanced choline and β-catenin pathway in breast cancer? A pilot study by MR Spectroscopy and ELISA. pubmed:28533512 |
|
Life Sciences |
Hepatoprotective effect of hesperidin in hepatocellular carcinoma: Involvement of Wnt signaling pathways. pubmed:28754618 |
|
Scientific Reports |
Is there an association between enhanced choline and β-catenin pathway in breast cancer? A pilot study by MR Spectroscopy and ELISA Pubmed: 28533512 |
|
The Showa University Journal of Medical Sciences |
Eribulin Treatment Induces High Expression of miR-195 and Inactivates the Wnt/β-catenin Signaling Pathway in Triple-negative Breast Cancer Doi: 10.15369/sujms.30.359 |
|
Advances in Medical Sciences |
Urinary procollagen III aminoterminal propeptide and β-catenin–New diagnostic biomarkers in solitary functioning kidney? Pubmed: 30738287 |
|
Biomed Pharmacother |
Silence of FAM83H-AS1 promotes chemosensitivity of gastric cancer through Wnt/β-catenin signaling pathway Pubmed: 32028241 |
|
Journal of Cosmetic Dermatology |
Novel complex of cosmetic ingredients with promising action in preventing hair loss and follicular aging through mechanism involving enrichment of WNT/signaling … Pubmed: 33179848 |
|
Nutrients |
Hydroxytyrosol as a Promising Ally in the Treatment of Fibromyalgia Pubmed: 32784915 |
|
Oncotarget |
Amalgamation of PI3K and EZH2 blockade synergistically regulates invasion and angiogenesis: combination therapy for glioblastoma multiforme 33473259 |
|
Cytokine |
QNZ alleviated hepatocellular carcinoma by targeting inflammatory pathways in a rat model 34564023 |
|
Catalog No. |
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Applications (RESEARCH USE ONLY!) |
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Main materials for "Do It(ELISA Kit) Yourself" |
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