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SKU:SEA025Ra
ELISA Kit for Eosinophil Chemotactic Factor (ECF)
ELISA Kit for Eosinophil Chemotactic Factor (ECF)
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Enzyme-linked immunosorbent assay for Antigen Detection.
Product No.
SEA025Ra
Organism Species
Rattus norvegicus (Rat).
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Test Method
Double-antibody Sandwich
Assay Length
3h
Detection Range
31.2-2,000pg/mL
Sensitivity
The minimum detectable dose of this kit is typically less than 12.5pg/mL.
UOM
48T 96T 96T*5 96T*10 96T*100
Specificity
This assay has high sensitivity and excellent specificity for detection of Eosinophil Chemotactic Factor (ECF).
No significant cross-reactivity or interference between Eosinophil Chemotactic Factor (ECF) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Eosinophil Chemotactic Factor (ECF) and the recovery rates were calculated by comparing the measured value to the expected amount of Eosinophil Chemotactic Factor (ECF) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 95-105 | 101 |
| EDTA plasma(n=5) | 81-94 | 85 |
| heparin plasma(n=5) | 80-91 | 85 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Eosinophil Chemotactic Factor (ECF) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Eosinophil Chemotactic Factor (ECF) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Eosinophil Chemotactic Factor (ECF) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 91-99% | 95-104% | 98-105% | 80-101% |
| EDTA plasma(n=5) | 82-96% | 80-89% | 82-104% | 91-105% |
| heparin plasma(n=5) | 80-92% | 97-104% | 88-101% | 79-90% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
| Standard | 2 | Standard Diluent | 1×20mL |
| Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
| Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
| TMB Substrate | 1×9mL | Stop Solution | 1×6mL |
| Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
| Magazine | Citations |
| INTERNATIONAL ARCHIVES OF ALLERGY AND IMMUNOLOGY | Elevated Serum Level of CD48 in Patients with Intermittent Allergic Rhinitis Pubmed: 32966985 |
| Exp Mol Pathol | The regulatory role of SFRP5/WNT5A axis in allergic rhinitis through inhibiting JNK pathway activation and lowering mucin generation in human nasal?¡ 33285209 |
| Immunopharmacol Immunotoxicol | sCD48 is elevated in non-allergic but not in allergic persistent rhinitis 34477021 |
| Exp Eye Res | Sulforaphane inhibits cytokine-stimulated chemokine and adhesion molecule expressions in human corneal fibroblasts: Involvement of the MAPK, STAT, and NF-κB … Pubmed:35038457 |
| Catalog No. | Related products for research use of Rattus norvegicus (Rat) Organism species | Applications (RESEARCH USE ONLY!) |
| RPA025Ra01 | Recombinant Eosinophil Chemotactic Factor (ECF) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA025Ra01 | Polyclonal Antibody to Eosinophil Chemotactic Factor (ECF) | WB; IHC; ICC; IP. |
| MAA025Ra21 | Monoclonal Antibody to Eosinophil Chemotactic Factor (ECF) | WB; IHC; ICC; IP. |
| SEA025Ra | ELISA Kit for Eosinophil Chemotactic Factor (ECF) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCA025Ra | CLIA Kit for Eosinophil Chemotactic Factor (ECF) | Chemiluminescent immunoassay for Antigen Detection. |
| LMA025Ra | Magnetic Luminex Assay Kit for Eosinophil Chemotactic Factor (ECF) ,etc. | Magnetic Luminex Assay for Antigen Detection. |
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