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SKU:SEB479Mu
ELISA Kit for Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF)
ELISA Kit for Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF)
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Enzyme-linked immunosorbent assay for Antigen Detection.
Product No.
SEB479Mu
Organism Species
Mus musculus (Mouse).
Sample Type
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Test Method
Double-antibody Sandwich
Assay Length
3h
Detection Range
15.6-1,000pg/mL
Sensitivity
The minimum detectable dose of this kit is typically less than 5.5pg/mL.
UOM
48T 96T 96T*5 96T*10 96T*100
Specificity
This assay has high sensitivity and excellent specificity for detection of Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF).
No significant cross-reactivity or interference between Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF) and the recovery rates were calculated by comparing the measured value to the expected amount of Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 78-97 | 88 |
| EDTA plasma(n=5) | 87-101 | 94 |
| heparin plasma(n=5) | 95-102 | 99 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 85-101% | 78-90% | 88-101% | 97-104% |
| EDTA plasma(n=5) | 92-105% | 87-101% | 79-96% | 80-90% |
| heparin plasma(n=5) | 78-94% | 82-93% | 79-95% | 81-91% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
| Standard | 2 | Standard Diluent | 1×20mL |
| Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
| Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
| TMB Substrate | 1×9mL | Stop Solution | 1×6mL |
| Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
| Magazine | Citations |
| Neuropathology | Astrocyte ERK phosphorylation precedes K+-induced swelling but follows hypotonicity-induced swelling. PubMed: 21118399 |
| Toxicological Sciences | Cypermethrin Induces Astrocyte Apoptosis by the Disruption of the Autocrine/Paracrine Mode of Epidermal Growth Factor Receptor Signaling Pubmed: source |
| PAIN RESEARCH | 軽度炎症性膀胱痛マウスモデルの構築とNGFの関与 Jstage:Source |
| European Journal of Pain | A novel method for assessing bladder-related pain reveals the involvement of nerve growth factor in painassociated with cyclophosphamide-induced chronic cystitis in mice. Pubmed:25820250 |
| Kinki daigaku | 2 型糖尿病モデルラットにおける糖尿病性腎症の進行とHB-EGF の関連性 17844 |
| laboratory investigation | Heparin-binding epidermal growth factor contributes to COPD disease severity by modulating airway fibrosis and pulmonary epithelial–mesenchymal transition Pubmed:29581578 |
| International Journal of Biological Sciences | CD9 regulates keratinocyte migration by negatively modulating the sheddase activity of ADAM17 |
| Redox Biology | Tumoral NOX4 recruits M2 tumor-associated macrophages via ROS/PI3K signaling-dependent various cytokine production to promote NSCLC growth Pubmed: 30769285 |
| JOURNAL OF CELLULAR AND MOLECULAR MEDICINE | Polarization of ADAM17‐driven EGFR signalling in electric field‐guided collective migration of epidermal sheets Pubmed: 33164313 |
| 象牙芽細胞の枯渇は象牙芽細胞分化と象牙質形成を誘導する |
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