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ELISA Kit for Terminal Complement Complex C5b-9 (C5b-9)
ELISA Kit for Terminal Complement Complex C5b-9 (C5b-9)
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Enzyme-linked immunosorbent assay for Antigen Detection.
Product No.
SEC350Mu
Organism Species
Mus musculus (Mouse).
Sample Type
serum, plasma and other biological fluids
Test Method
Double-antibody Sandwich
Assay Length
3h
Detection Range
1.56-100ng/mL
Sensitivity
The minimum detectable dose of this kit is typically less than 0.65ng/mL.
UOM
48T 96T 96T*5 96T*10 96T*100
Specificity
This assay has high sensitivity and excellent specificity for detection of Terminal Complement Complex C5b-9 (C5b-9).
No significant cross-reactivity or interference between Terminal Complement Complex C5b-9 (C5b-9) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Terminal Complement Complex C5b-9 (C5b-9) and the recovery rates were calculated by comparing the measured value to the expected amount of Terminal Complement Complex C5b-9 (C5b-9) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 93-101 | 96 |
| EDTA plasma(n=5) | 84-101 | 89 |
| heparin plasma(n=5) | 88-95 | 92 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Terminal Complement Complex C5b-9 (C5b-9) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Terminal Complement Complex C5b-9 (C5b-9) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Terminal Complement Complex C5b-9 (C5b-9) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 80-97% | 81-88% | 95-102% | 87-102% |
| EDTA plasma(n=5) | 88-105% | 79-97% | 89-96% | 95-102% |
| heparin plasma(n=5) | 82-91% | 87-101% | 99-105% | 97-105% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
| Standard | 2 | Standard Diluent | 1×20mL |
| Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
| Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
| TMB Substrate | 1×9mL | Stop Solution | 1×6mL |
| Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
| Magazine | Citations |
| Journal of Biomedical Materials Research Part A | Effect of thiol functionalization on the hemo-compatibility of PLGA nanoparticles Wiley: source |
| The Journal of Clinical Investigation | Complement component 5 contributes to poor disease outcome in humans and mice with pneumococcal meningitis PubMed: PMC3195471 |
| Colloids and Surfaces B: Biointerfaces | Glucosylated polymeric nanoparticles: A sweetened approach against blood compatibility paradox ScienceDirect: S0927776513001720 |
| Perfusion | The effect of normovolemic modified ultrafiltration on inflammatory mediators, endotoxins, terminal complement complexes and clinical outcome in high-risk cardiac surgery patients Pubmed: 23429100 |
| Nephrology Dialysis Transplantation | The efficacy of recombinant human soluble thrombomodulin for the treatment of shiga toxin associated hemolytic uremic syndrome model mice Pubmed:25694534 |
| journal of neuroinflammation | Adjuvant treatment with dexamethasone plus anti-C5 antibodies improves outcome of experimental pneumococcal meningitis: a randomized controlled trial PubMed: 26272468 |
| J Thromb Haemost | Thrombin‐activatable fibrinolysis inhibitor influences disease severity in humans and mice with pneumococcal meningitis PubMed: 26340319 |
| Digital Repository | Dosagem de frações ativadas do sistema complemento em empiema induzido em ratos 10183 |
| J Neuroinflammation. | Mannose-binding lectin-associated serine protease 2 (MASP-2) contributes to poor disease outcome in humans and mice with pneumococcal meningitis PMC5234106 |
| Cancer Letters | Complement C5a/C5aR pathway potentiates the pathogenesis Q5 of gastric cancer by down-regulating p21 expression pubmed:29031586 |
| Effects of immunoadsorption combined with membrane filtration on complement markers–Results of a randomized, controlled, crossover study | |
| Journal of Neuroinflammation | Complement factor H contributes to mortality in humans and mice with bacterial meningitis Pubmed: 31883521 |
| FASEB J | C‐reactive protein inhibits C3a/C3aR‐dependent podocyte autophagy in favor of diabetic kidney disease Pubmed:35503088 |
| Catalog No. | Related products for research use of Mus musculus (Mouse) Organism species | Applications (RESEARCH USE ONLY!) |
| SEC350Mu | ELISA Kit for Terminal Complement Complex C5b-9 (C5b-9) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMC350Mu | Magnetic Luminex Assay Kit for Terminal Complement Complex C5b-9 (C5b-9) ,etc. | Magnetic Luminex Assay for Antigen Detection. |
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