SKU:SEB171Ra
ELISA Kit for Transferrin Receptor (TFR)
ELISA Kit for Transferrin Receptor (TFR)
Enzyme-linked immunosorbent assay for Antigen Detection.
Product No.
SEB171Ra
Organism Species
Rattus norvegicus (Rat).
Sample Type
Serum, plasma and other biological fluids
Test Method
Double-antibody Sandwich
Assay Length
3h
Detection Range
0.625-40ng/mL
Sensitivity
The minimum detectable dose of this kit is typically less than 0.239ng/mL.
UOM
48T 96T 96T*5 96T*10 96T*100
Specificity
This assay has high sensitivity and excellent specificity for detection of Transferrin Receptor (TFR).
No significant cross-reactivity or interference between Transferrin Receptor (TFR) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Transferrin Receptor (TFR) and the recovery rates were calculated by comparing the measured value to the expected amount of Transferrin Receptor (TFR) in samples.
Matrix | Recovery range (%) | Average(%) |
serum(n=5) | 92-101 | 96 |
EDTA plasma(n=5) | 84-103 | 93 |
heparin plasma(n=5) | 83-103 | 94 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Transferrin Receptor (TFR) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Transferrin Receptor (TFR) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Transferrin Receptor (TFR) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 84-101% | 82-94% | 91-98% | 93-102% |
EDTA plasma(n=5) | 98-105% | 93-101% | 80-88% | 81-92% |
heparin plasma(n=5) | 94-101% | 86-101% | 79-95% | 90-102% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
Reagents | Quantity | Reagents | Quantity |
Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
Standard | 2 | Standard Diluent | 1×20mL |
Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
TMB Substrate | 1×9mL | Stop Solution | 1×6mL |
Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
Magazine | Citations |
British Journal of Nutrition | Survey of anaemia and Helicobacter pylori infection in adolescent girls in Suihua, China and enhancement of iron intervention effects by H. pylori eradication PubMed: 22004585 |
Food Funct | The effects of polysaccharides from the root of Angelica sinensis on tumor growth and iron metabolism in H22-bearing mice Pubmed:26757699 |
Physiological research | The Labile Iron Pool in Monocytes Reflects the Activity of the Atherosclerotic Process in Men with Chronic Cardiovascular Disease pubmed:27782743 |
Clinical and Experimental Medicine | Increased NGAL level associated with iron store in chronic kidney disease with anemia Pubmed:29909502 |
J Mol Cell Cardiol | Systemic iron deficiency does not affect the cardiac iron content and progression of heart failure 34139233 |
Catalog No. | Related products for research use of Rattus norvegicus (Rat) Organism species | Applications (RESEARCH USE ONLY!) |
RPB171Ra01 | Recombinant Transferrin Receptor (TFR) | Positive Control; Immunogen; SDS-PAGE; WB. |
PAB171Ra01 | Polyclonal Antibody to Transferrin Receptor (TFR) | WB; IHC; ICC; IP. |
MAB171Ra21 | Monoclonal Antibody to Transferrin Receptor (TFR) | WB; IHC; ICC; IP. |
SEB171Ra | ELISA Kit for Transferrin Receptor (TFR) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMB171Ra | Magnetic Luminex Assay Kit for Transferrin Receptor (TFR) ,etc. | Magnetic Luminex Assay for Antigen Detection. |